AB180123

Anti-Peroxiredoxin 5 antibody [EPR14528(B)]

RabMAb
Recombinant
KO Validated
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Peroxiredoxin 5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ACR1, SBBI10, PRDX5, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, AOEB166, Prx-V

5 Images

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5with ab180123 at 1/10 dilution (red) compared toa rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Predicted band size: 22 kDa

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Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

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Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : PRDX5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HepG2 whole cell lysate (20 μg)
Lane 4 : A549 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Predicted band size: 22 kDa

false

Carrier free

Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14528(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 5 (Prdx5) acts as a peroxidase reducing hydrogen peroxide and organic hydroperoxides and protecting cells from oxidative damage. This protein also known as AOEB166 and PRDX6 has a molecular mass of approximately 17 kDa. Prdx5 is widely expressed across various tissues including lung liver heart kidney and brain where it performs protective and regulatory roles. It localizes within multiple cell compartments such as mitochondria peroxisomes and the cytosol.

Biological function summary

Prdx5 plays a role in reducing oxidative stress which helps to maintain cellular homeostasis. It is part of the large family of peroxiredoxins known for their antioxidant activity. Prdx5 interacts with other antioxidants within the cell to mitigate damage from reactive oxygen species (ROS). Its multifunctionality allows it to act both as an antioxidant and a molecular chaperone under certain stress conditions helping cells adapt to challenging environments.

Pathways

Prdx5 functions within the antioxidant defense system and participates in the regulatory networks that control oxidative stress responses. It is an element of the thioredoxin system working alongside thioredoxin and thioredoxin reductase to neutralize ROS. Additionally it contributes to the signaling pathways of apoptosis by controlling ROS levels which can impact cell viability and proliferation.

The malfunction or dysregulation of Prdx5 associates with cancer and neurodegenerative diseases. Elevated ROS levels can contribute to tumor progression and have been observed in cancerous tissues where Prdx5 may have altered expression. In neurodegeneration such as Alzheimer's disease Prdx5's role in oxidative stress management potentially affects disease progression. Connections exist between Prdx5 and proteins like amyloid-beta in Alzheimer's illustrating its importance in both protective and pathological contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

See full target information PRDX5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:334 PubMed40274778

2025

The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Liu,Ying Lu,Runye Zhu,Ping Xi,Zhihao Yang,Zhipeng Zhang,Yunbing Xiong,Yifu Liu,Qiqi Zhu,Ting Sun,Wenjie Xie,Binbin Gong

Basic research in cardiology 118:15 PubMed37138037

2023

Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Richard T Clements,Radmila Terentyeva,Shanna Hamilton,Paul M L Janssen,Karim Roder,Benjamin Y Martin,Fruzsina Perger,Timothy Schneider,Zuzana Nichtova,Anindhya S Das,Roland Veress,Beth S Lee,Do-Gyoon Kim,Gideon Koren,Matthew S Stratton,Gyorgy Csordas,Federica Accornero,Andriy E Belevych,Sandor Gyorke,Dmitry Terentyev

Redox biology 30:101417 PubMed31901729

2020

Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Lovatt,Khadijah Adnan,Viridiana Kocaba,Martin Dirisamer,Gary S L Peh,Jodhbir S Mehta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Peroxiredoxin 5 antibody [EPR14528(B)]

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (AB180123)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise