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AB250136

Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Peroxiredoxin 5 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ACR1, SBBI10, PRDX5, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, AOEB166, Prx-V

5 Images
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)
  • IP

Supplier Data

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>)

Predicted band size: 22 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)
  • WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : PRDX5 knockout HAP1 whole cell lysate (20 μg)

Lane 3 : HepG2 whole cell lysate (20 μg)

Lane 4 : A549 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>)

Predicted band size: 22 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)
  • WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14528(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250136 is the carrier-free version of ab180123.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 5 (Prdx5) acts as a peroxidase reducing hydrogen peroxide and organic hydroperoxides and protecting cells from oxidative damage. This protein also known as AOEB166 and PRDX6 has a molecular mass of approximately 17 kDa. Prdx5 is widely expressed across various tissues including lung liver heart kidney and brain where it performs protective and regulatory roles. It localizes within multiple cell compartments such as mitochondria peroxisomes and the cytosol.
Biological function summary

Prdx5 plays a role in reducing oxidative stress which helps to maintain cellular homeostasis. It is part of the large family of peroxiredoxins known for their antioxidant activity. Prdx5 interacts with other antioxidants within the cell to mitigate damage from reactive oxygen species (ROS). Its multifunctionality allows it to act both as an antioxidant and a molecular chaperone under certain stress conditions helping cells adapt to challenging environments.

Pathways

Prdx5 functions within the antioxidant defense system and participates in the regulatory networks that control oxidative stress responses. It is an element of the thioredoxin system working alongside thioredoxin and thioredoxin reductase to neutralize ROS. Additionally it contributes to the signaling pathways of apoptosis by controlling ROS levels which can impact cell viability and proliferation.

The malfunction or dysregulation of Prdx5 associates with cancer and neurodegenerative diseases. Elevated ROS levels can contribute to tumor progression and have been observed in cancerous tissues where Prdx5 may have altered expression. In neurodegeneration such as Alzheimer's disease Prdx5's role in oxidative stress management potentially affects disease progression. Connections exist between Prdx5 and proteins like amyloid-beta in Alzheimer's illustrating its importance in both protective and pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
See full target information PRDX5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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