Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>)

Predicted band size: 22 kDa

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• WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : PRDX5 knockout HAP1 whole cell lysate (20 μg)

Lane 3 : HepG2 whole cell lysate (20 μg)

Lane 4 : A549 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>)

Predicted band size: 22 kDa

false

• WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (AB250136)

This data was developed using ab180123, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-5-antibody-epr14528b-ab180123'>ab180123</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false