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AB180587

Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(13 Publications)

Rabbit Recombinant Monoclonal Peroxiredoxin 5 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

ACR1, SBBI10, PRDX5, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, AOEB166, Prx-V

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1 : 500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • IP

Unknown

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

ab180587 (Purified) at 1 : 30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab180587 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab180587 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Predicted band size: 22 kDa

false

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • IP

Supplier Data

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution

All lanes:

HeLa cell immunoprecipitated with ab180587 at 1/50

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa,50 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : PRDX5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HepG2 whole cell lysate (20 μg)
Lane 4 : A549 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180587 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Predicted band size: 22 kDa,44 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

Lane 1:

HepG2 cell line lysate at 20 µg

Lane 2:

Jurkat cell line lysate at 20 µg

Lane 3:

A549 cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution

All lanes:

HeLa cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)
  • WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

All lanes:

Mouse brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

  • Carrier free

    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] - BSA and Azide free

  • 578 PE

    PE Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 660 APC

    APC Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14529(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 5 (Prdx5) acts as a peroxidase reducing hydrogen peroxide and organic hydroperoxides and protecting cells from oxidative damage. This protein also known as AOEB166 and PRDX6 has a molecular mass of approximately 17 kDa. Prdx5 is widely expressed across various tissues including lung liver heart kidney and brain where it performs protective and regulatory roles. It localizes within multiple cell compartments such as mitochondria peroxisomes and the cytosol.
Biological function summary

Prdx5 plays a role in reducing oxidative stress which helps to maintain cellular homeostasis. It is part of the large family of peroxiredoxins known for their antioxidant activity. Prdx5 interacts with other antioxidants within the cell to mitigate damage from reactive oxygen species (ROS). Its multifunctionality allows it to act both as an antioxidant and a molecular chaperone under certain stress conditions helping cells adapt to challenging environments.

Pathways

Prdx5 functions within the antioxidant defense system and participates in the regulatory networks that control oxidative stress responses. It is an element of the thioredoxin system working alongside thioredoxin and thioredoxin reductase to neutralize ROS. Additionally it contributes to the signaling pathways of apoptosis by controlling ROS levels which can impact cell viability and proliferation.

The malfunction or dysregulation of Prdx5 associates with cancer and neurodegenerative diseases. Elevated ROS levels can contribute to tumor progression and have been observed in cancerous tissues where Prdx5 may have altered expression. In neurodegeneration such as Alzheimer's disease Prdx5's role in oxidative stress management potentially affects disease progression. Connections exist between Prdx5 and proteins like amyloid-beta in Alzheimer's illustrating its importance in both protective and pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
See full target information PRDX5
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:533 PubMed40122834

2025

Single-cell sequencing combined with urinary multi-omics analysis reveals that the non-invasive biomarker PRDX5 regulates bladder cancer progression through ferroptosis signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Wan,Kun-Peng Li,Si-Yu Chen,Chen-Yang Wang,Kun Cheng,Jian-Wei Yang,Li-Yun Ding,Tuan-Jie Che,Shan-Hui Liu,Li Yang

Frontiers in reproductive health 6:1479568 PubMed39726694

2024

Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Raneen Sawaid Kaiyal,Sromona D Mukherjee,Manesh Kumar Panner Selvam,Aaron W Miller,Sarah C Vij,Scott D Lundy

International journal of molecular sciences 25: PubMed38892076

2024

Anti-HMGB1 mAb Therapy Reduces Epidural Hematoma Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shangze Gao,Dengli Wang,Keyue Liu,Yasuko Tomono,Li Fu,Yuan Gao,Yohei Takahashi,Mariko Yata,Masahiro Nishibori

Molecular cell 83:3931-3939.e5 PubMed37863053

2023

Identification of hyperoxidized PRDX3 as a ferroptosis marker reveals ferroptotic damage in chronic liver diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojie Cui,Anchal Ghai,Yaqin Deng,Shili Li,Ruihui Zhang,Christopher Egbulefu,Guosheng Liang,Samuel Achilefu,Jin Ye

British journal of pharmacology 180:647-666 PubMed36321732

2022

Gut microbiota-derived nicotinamide mononucleotide alleviates acute pancreatitis by activating pancreatic SIRT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Wei Liu,Yu Xie,Guan-Qun Li,Tao Zhang,Yu-Hang Sui,Zhong-Jie Zhao,Yang-Yang Zhang,Wen-Bo Yang,Xing-Long Geng,Dong-Bo Xue,Hua Chen,Yong-Wei Wang,Tian-Qi Lu,Li-Ren Shang,Zhi-Bo Li,Le Li,Bei Sun

International journal of molecular sciences 23: PubMed36362154

2022

Proteomic Profiling Revealed Mitochondrial Dysfunction in Photoreceptor Cells under Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Christie Hang-I Lam,Jimmy Ka-Wai Cheung,Dennis Yan-Yin Tse,Thomas Chuen Lam

Nature communications 12:6626 PubMed34785665

2021

Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael F Queiroz,Christopher P Stanley,Kathryn Wolhuter,Stephanie M Y Kong,Ragul Rajivan,Naomi McKinnon,Giang T H Nguyen,Antonella Roveri,Sebastian Guttzeit,Philip Eaton,William A Donald,Fulvio Ursini,Christine C Winterbourn,Anita Ayer,Roland Stocker

International journal of molecular medicine 47:573-582 PubMed33416106

2021

ROS‑mediated hypomethylation of PRDX5 promotes STAT3 binding and activates the Nrf2 signaling pathway in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Cao,Xin-Ming Chen,Wei-Zhang Xiao,Ben Li,Bo Zhang,Qiong Wu,Qun Xue

Redox biology 28:101345 PubMed31669973

2019

Metformin protects against PM-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2.

Applications

Unspecified application

Species

Unspecified reactive species

Junling Gao,Juntao Yuan,Qiao'e Wang,Tong Lei,Xiyue Shen,Bingqing Cui,Fang Zhang,Wenjun Ding,Zhongbing Lu

Molecular and cellular biochemistry 461:91-102 PubMed31375973

2019

A key metabolic integrator, coenzyme A, modulates the activity of peroxiredoxin 5 via covalent modification.

Applications

Unspecified application

Species

Unspecified reactive species

Jovana Baković,Bess Yi Kun Yu,Daniel Silva,Sew Peak Chew,Sangeun Kim,Sun-Hee Ahn,Laura Palmer,Lujain Aloum,Giacomo Stanzani,Oksana Malanchuk,Michael R Duchen,Mervyn Singer,Valeriy Filonenko,Tae-Hoon Lee,Mark Skehel,Ivan Gout
View all publications
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Product promise

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