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Rabbit Recombinant Monoclonal Peroxiredoxin 5 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

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Images

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587), expandable thumbnail
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/250
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

Alternative names

ACR1, SBBI10, PRDX5, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, AOEB166, Prx-V

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 5 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14529(B)
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Peroxiredoxin 5 (Prdx5) acts as a peroxidase reducing hydrogen peroxide and organic hydroperoxides and protecting cells from oxidative damage. This protein also known as AOEB166 and PRDX6 has a molecular mass of approximately 17 kDa. Prdx5 is widely expressed across various tissues including lung liver heart kidney and brain where it performs protective and regulatory roles. It localizes within multiple cell compartments such as mitochondria peroxisomes and the cytosol.

Biological function summary

Prdx5 plays a role in reducing oxidative stress which helps to maintain cellular homeostasis. It is part of the large family of peroxiredoxins known for their antioxidant activity. Prdx5 interacts with other antioxidants within the cell to mitigate damage from reactive oxygen species (ROS). Its multifunctionality allows it to act both as an antioxidant and a molecular chaperone under certain stress conditions helping cells adapt to challenging environments.

Pathways

Prdx5 functions within the antioxidant defense system and participates in the regulatory networks that control oxidative stress responses. It is an element of the thioredoxin system working alongside thioredoxin and thioredoxin reductase to neutralize ROS. Additionally it contributes to the signaling pathways of apoptosis by controlling ROS levels which can impact cell viability and proliferation.

Associated diseases and disorders

The malfunction or dysregulation of Prdx5 associates with cancer and neurodegenerative diseases. Elevated ROS levels can contribute to tumor progression and have been observed in cancerous tissues where Prdx5 may have altered expression. In neurodegeneration such as Alzheimer's disease Prdx5's role in oxidative stress management potentially affects disease progression. Connections exist between Prdx5 and proteins like amyloid-beta in Alzheimer's illustrating its importance in both protective and pathological contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    ab180587 (Purified) at 1:30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab180587 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab180587 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Predicted band size: 22 kDa

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)
    Lane 4: A549 whole cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180587 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Predicted band size: 22 kDa, 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1:500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.

    All lanes: Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution

    All lanes: HeLa cell immunoprecipitated with ab180587 at 1/50

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 17 kDa, 50 kDa

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    All lanes: Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: Human brain lysates at 20 µg

    Lane 3: Rat brain lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    All lanes: Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

    All lanes: Mouse brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    All lanes: Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

    Lane 1: HepG2 cell line lysate at 20 µg

    Lane 2: Jurkat cell line lysate at 20 µg

    Lane 3: A549 cell line lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    All lanes: Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution

    All lanes: HeLa cell line lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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