Rabbit Recombinant Monoclonal Peroxiredoxin 6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445954.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916)")
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445953.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916)")
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445952.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (AB323916)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (PubMed:10893423, PubMed:9497358). Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides (PubMed:10893423). Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (PubMed:10893423, PubMed:26830860). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (PubMed:10893423). Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (PubMed:10893423). Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:26830860). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (PubMed:26830860).
Alternative names
AOP2, KIAA0106, PRDX6, Peroxiredoxin-6, 1-Cys peroxiredoxin, 24 kDa protein, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Glutathione-dependent peroxiredoxin, Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5, NSGPx
Rabbit Recombinant Monoclonal Peroxiredoxin 6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26596-61
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323916 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Peroxiredoxin 6. The recommended pair for sandwich ELISA is:
Capture: ab323916, Human Peroxiredoxin 6 Capture Antibody (unconjugated)
Detector: Anti-Peroxiredoxin 6 antibody [EPR26596-41] - BSA and Azide free (Detector) ab323917, Human Peroxiredoxin 6 Detector Antibody (unconjugated)
The reference range value is 78.1-5000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445954.jpg "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)")
Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)
Interpolated concentration of native Peroxiredoxin 6 was measured at different sample concentrations. Undiluted sample is as follows: HAP1 cell extract (500 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445953.jpg "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)")
Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)
Interpolated concentrations of native Peroxiredoxin 6 in human control wild type HAP1 (human near-haploid cell) and PRDX6 (Peroxiredoxin 6) knockout HAP1 cell based on 500 μg/mL extract loads. The concentrations of Peroxiredoxin 6 were measured in duplicate and interpolated from the Peroxiredoxin 6 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Peroxiredoxin 6 concentration was determined to be 2070 pg/mL in wild type HAP1 extract and undetectable in PRDX6 (Peroxiredoxin 6) knockout HAP1 extract.
![Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-6-antibody-epr26596-61-bsa-and-azide-free-capture-ab323916--sandwich-elisa-img445952.jpg "Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)")
Sandwich ELISA - Anti-Peroxiredoxin 6 antibody [EPR26596-61] - BSA and Azide free (Capture) (ab323916)
Sandwich ELISA with capture antibody ab323916 dilution at 2 μg/mL and detector antibody Anti-Peroxiredoxin 6 antibody [EPR26596-41] - BSA and Azide free (Detector) ab323917 dilution at 0.5 μg/mL.
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