Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348). Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1 - 6:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-6-antibody-epr3754-ab133348'>ab133348</a>) at 1/1000 dilution

Lanes 1 - 6:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Lane 1:

Mouse liver whole cell lysate at 20 µg

Lane 2:

Mouse testis whole cell lysate at 20 µg

Lane 3:

Mouse spleen whole cell lysate at 20 µg

Lane 4:

Rat liver whole cell lysate at 20 µg

Lane 5:

Rat testis whole cell lysate at 20 µg

Lane 6:

Rat spleen whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-6-antibody-epr3754-ab133348'>ab133348</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

A431 whole cell lysate at 20 µg

Lane 4:

PC3 whole cell lysate at 20 µg

Predicted band size: 25 kDa

false

• WB

Collaborator

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (AB240057)

This data was developed using the same antibody in a different buffer formulation (ab133348).

ab133348 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133348 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-6-antibody-epr3754-ab133348'>ab133348</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

PRDX6 knockout HAP1 cell lysate at 20 µg

Predicted band size: 25 kDa

false