Anti-Peroxiredoxin 6 antibody [EPR3755]

• WB

Unknown

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3755] (AB92322)

All lanes:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3755] (ab92322) at 1/500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 25 kDa

false

• IP

Collaborator

Immunoprecipitation - Anti-Peroxiredoxin 6 antibody [EPR3755] (AB92322)

Immunoprecipitation of PRDX6 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0μg of ab92322 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab133348 at 1/1000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 6 antibody [EPR3755] (ab92322)

Predicted band size: 25 kDa

false

• WB

Collaborator

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3755] (AB92322)

ab92322 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab92322 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3755] (ab92322) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

PRDX6 knockout HAP1 cell lysate at 20 µg

Predicted band size: 25 kDa

false