Rabbit Polyclonal PERP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human PERP aa 150 to C-terminus.
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- Free radical biology & medicine 167:307-3202021Novel function of PERP-428 variants impacts lung cancer risk through the differential regulation of PTEN/MDM2/p53-mediated antioxidant activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen-Yi Liao,Shun-Fa Yang,Ting-Jian Wu,Han Chang,Chi-Ying F Huang,Yu-Fan Liu,Chi-Hsiang Wang,Jhong-Chio Liou,Shih-Lan Hsu,Huei Lee,Gwo-Tarng Sheu,Jinghua Tsai ChangPubMed 33731308
- Breast cancer research : BCR 22:1052020Trastuzumab-induced upregulation of a protein set in extracellular vesicles emitted by ErbB2-positive breast cancer cells correlates with their trastuzumab sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArik Drucker et. al.PubMed 33023655
- Cell death discovery 5:1322019ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha J McDonnell et. al.PubMed 31508245
- British journal of cancer 115:983-9922016p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma.
- Oncogene 35:5759-57692016ErbB2-dependent downregulation of a pro-apoptotic protein Perp is required for oncogenic transformation of breast epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI A Khan et. al.PubMed 27109096
- BioMed research international 2015:7319382015Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts.
- Journal of neuro-oncology 117:183-1892014High expression of the stem cell marker nestin is an adverse prognostic factor in WHO grade II-III astrocytomas and oligoastrocytomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimmo J Hatanpaa,Tianshen Hu,Vamsidhara Vemireddy,Chan Foong,Jack M Raisanen,Dwight Oliver,Matthew C Hiemenz,Dennis K Burns,Charles L White,L Anthony Whitworth,Bruce Mickey,Martha Stegner,Amyn A Habib,Karen Fink,Elizabeth A Maher,Robert M BachooPubMed 24519516
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 40:67-762010A molecular study of desmosomes identifies a desmoglein isoform switch in head and neck squamous cell carcinoma.Applications:WBReactive species:HumanMuy-Teck Teh,E Ken Parkinson,Johanna K Thurlow,Feng Liu,Farida Fortune,Hong WanPubMed 20923451
- Molecular vision 14:2031-412008Preferential gene expression in the limbus of the vervet monkey.
- Experimental eye research 83:911-92006Expression of p53-induced apoptosis effector PERP in primary uveal melanomas: downregulation is associated with aggressive type.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuminita Paraoan et. al.PubMed 16784742
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PERP aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96FX8
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Component of intercellular desmosome junctions (By similarity). Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly (By similarity). Thereby plays a role in barrier function of the skin against infection (By similarity). Plays a role in mammary epithelial tissue homeostasis and remodeling during and after pregnancy, potentially via its involvement in desmosome cell-cell junctions (By similarity). Required for tooth enamel development via facilitating desmosome-mediated ameloblast adhesion to the stratum intermedium during the transitional stage of amelogenesis (By similarity). May also play a role in downstream transcriptional regulation of other genes involved in amelogenesis such as AMBN, ENAM, MMP20 and KLK4 (By similarity). Plays a role as an effector in the TP53-dependent apoptotic pathway (By similarity). Positively regulates apoptosis in T-helper 17 (Th17) cell populations via caspase-dependent signaling (By similarity). Promotes neutrophil transepithelial migration in response to chemoattractants such as hepoxilin A3 (HXA3), N-Formylmethionyl-leucyl-phenylalanine (fMLP) and CXCL8/IL-8 (PubMed:25486861). Required for neutrophil transepithelial migration in response to S.typhimurium infection (PubMed:25486861). May act as a positive regulator of endothelial cell apoptosis in response to blood flow-derived sheer stress (By similarity).
Alternative names
KCP1, KRTCAP1, PIGPC1, THW, PERP, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, P53-induced protein PIGPC1, Transmembrane protein THW, KCP-1
Recommended products
Rabbit Polyclonal PERP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human PERP aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PERP aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96FX8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PERP also known as P53 apoptosis effector related to PMP-22 is a protein with an approximate mass of 23 kDa. It mainly operates as an effector in the p53-dependent apoptosis pathway. PERP is known for facilitating cell-cell adhesion as a part of the desmosome complex. Researchers have found its expression primarily in epithelial tissues where it contributes to maintaining cell structure integrity. PERP's role is significant in tissues experiencing stress where it upregulates in response to p53 activation.
- Biological function summary
PERP plays an active role in cellular processes like adhesion and apoptosis. As a part of the desmosome it helps in connecting epithelial cells. This function is important for preserving tissue architecture and responding to mechanical stress. PERP's involvement in apoptosis arises from its interactions in the p53 pathway supporting the process when cells require programmed death. These roles are essential for maintaining balanced cell proliferation and death key for tissue homeostasis.
- Pathways
PERP is closely tied to the p53 pathway and the desmosomal adhesion process. The p53 pathway involves one of the primary tumor suppressor proteins and regulates the cell cycle while the desmosomal adhesion pathway maintains the structural cohesion between cells. PERP acts in concert with various proteins such as JUP (Plakoglobin) strengthening cellular adhesions and responding to apoptotic signals. This connectivity allows PERP to perform dual roles in maintaining cell stability and facilitating apoptosis when necessary.
- Associated diseases and disorders
PERP has associations with squamous cell carcinoma and epidermolysis bullosa. Disruption of PERP functions can lead to improper apoptosis contributing to cancer progression. In the case of epidermolysis bullosa alterations in desmosomal structure connected to PERP can weaken skin integrity leading to blistering. In these conditions proteins like JUP work alongside PERP highlighting the importance of balance between cell adhesion and controlled cell death in disease prevention.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-PERP antibody (ab5986)
All lanes: Western blot - Anti-PERP antibody (ab5986) at 1 µg
Lane 1: 293 cell lysate at 15 µg
Lane 2: A549 cell lysate at 15 µg
Lane 3: HeLa cell lysate at 15 µg
Lane 4: HepG2 cell lysate at 15 µg
Lane 5: K569 cell lysate at 15 µg
Lane 6: MCF-7 cell lysate at 15 µg
Lane 7: SK-N-SH cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 21 kDa
Western blot - Anti-PERP antibody (ab5986)
Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 μg /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml.
All lanes: Western blot - Anti-PERP antibody (ab5986)
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody (ab5986)
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-PERP antibody (ab5986)
PERP western blot using anti-PERP antibody ab5986. Publication image and figure legend from Marthandan, S., Priebe, S., et al., 2015, Biomed Res Int, PubMed 26339636.
ab5986 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5986 please see the product overview.
Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured with RNA-seq and Western Blots. (a) 8 genes commonly downregulated and (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first and the last PD investigated here (RNA-seq: log 2 RPKM FC; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterix: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots.
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