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Rabbit Recombinant Monoclonal PERP antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-PERP antibody [EPR7885] (AB129083), expandable thumbnail
  • Western blot - Anti-PERP antibody [EPR7885] (AB129083), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (AB129083), expandable thumbnail
  • Western blot - Anti-PERP antibody [EPR7885] (AB129083), expandable thumbnail
  • OI-RD Scanning - Anti-PERP antibody [EPR7885] (AB129083), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Expected
Rat
Not recommended
Not recommended
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of intercellular desmosome junctions (By similarity). Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly (By similarity). Thereby plays a role in barrier function of the skin against infection (By similarity). Plays a role in mammary epithelial tissue homeostasis and remodeling during and after pregnancy, potentially via its involvement in desmosome cell-cell junctions (By similarity). Required for tooth enamel development via facilitating desmosome-mediated ameloblast adhesion to the stratum intermedium during the transitional stage of amelogenesis (By similarity). May also play a role in downstream transcriptional regulation of other genes involved in amelogenesis such as AMBN, ENAM, MMP20 and KLK4 (By similarity). Plays a role as an effector in the TP53-dependent apoptotic pathway (By similarity). Positively regulates apoptosis in T-helper 17 (Th17) cell populations via caspase-dependent signaling (By similarity). Promotes neutrophil transepithelial migration in response to chemoattractants such as hepoxilin A3 (HXA3), N-Formylmethionyl-leucyl-phenylalanine (fMLP) and CXCL8/IL-8 (PubMed:25486861). Required for neutrophil transepithelial migration in response to S.typhimurium infection (PubMed:25486861). May act as a positive regulator of endothelial cell apoptosis in response to blood flow-derived sheer stress (By similarity).

Alternative names

KCP1, KRTCAP1, PIGPC1, THW, PERP, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, P53-induced protein PIGPC1, Transmembrane protein THW, KCP-1

Recommended products

Rabbit Recombinant Monoclonal PERP antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7885
Purification technique
Affinity purification Protein A
Dissociation constant
4.1 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PERP also known as P53 apoptosis effector related to PMP-22 is a protein with an approximate mass of 23 kDa. It mainly operates as an effector in the p53-dependent apoptosis pathway. PERP is known for facilitating cell-cell adhesion as a part of the desmosome complex. Researchers have found its expression primarily in epithelial tissues where it contributes to maintaining cell structure integrity. PERP's role is significant in tissues experiencing stress where it upregulates in response to p53 activation.

Biological function summary

PERP plays an active role in cellular processes like adhesion and apoptosis. As a part of the desmosome it helps in connecting epithelial cells. This function is important for preserving tissue architecture and responding to mechanical stress. PERP's involvement in apoptosis arises from its interactions in the p53 pathway supporting the process when cells require programmed death. These roles are essential for maintaining balanced cell proliferation and death key for tissue homeostasis.

Pathways

PERP is closely tied to the p53 pathway and the desmosomal adhesion process. The p53 pathway involves one of the primary tumor suppressor proteins and regulates the cell cycle while the desmosomal adhesion pathway maintains the structural cohesion between cells. PERP acts in concert with various proteins such as JUP (Plakoglobin) strengthening cellular adhesions and responding to apoptotic signals. This connectivity allows PERP to perform dual roles in maintaining cell stability and facilitating apoptosis when necessary.

Associated diseases and disorders

PERP has associations with squamous cell carcinoma and epidermolysis bullosa. Disruption of PERP functions can lead to improper apoptosis contributing to cancer progression. In the case of epidermolysis bullosa alterations in desmosomal structure connected to PERP can weaken skin integrity leading to blistering. In these conditions proteins like JUP work alongside PERP highlighting the importance of balance between cell adhesion and controlled cell death in disease prevention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PERP antibody [EPR7885] (ab129083), expandable thumbnail

    Western blot - Anti-PERP antibody [EPR7885] (ab129083)

    False colour image of Western blot: Anti-PERP antibody [EPR7885] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab129083 was shown to bind specifically to PERP. A band was observed at 17 kDa in wild-type A431 cell lysates with no signal observed at this size in PERP knockout cell line Human PERP knockout A-431 cell line ab270475 (knockout cell lysate Human PERP knockout A-431 cell lysate ab270498). To generate this image, wild-type and PERP knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: PERP knockout A431 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: MOLT-4 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-PERP antibody [EPR7885] (ab129083), expandable thumbnail

    Western blot - Anti-PERP antibody [EPR7885] (ab129083)

    Lanes 1-3: Merged signal (red and green). Green - ab129083 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab129083 Anti-PERP antibody [EPR7885] was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PERP knockout HeLa cell line ab265335 (knockout cell lysate Human PERP knockout HeLa cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PERP knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human PERP knockout HeLa cell line (Human PERP knockout HeLa cell line ab265335)

    Lane 3: HaCaT cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)

    ab129083, at a dilution of 1/100, staining PERP in A431 cells by Immunofluorescence.

  • Western blot - Anti-PERP antibody [EPR7885] (ab129083), expandable thumbnail

    Western blot - Anti-PERP antibody [EPR7885] (ab129083)

    All lanes: Western blot - Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution

    Lane 1: Mouse heart lysate at 10 µg

    Lane 2: Rat heart lysate at 10 µg

    Lane 3: A431 lysate at 10 µg

    Lane 4: HACAT lysate at 10 µg

    Lane 5: Human skin lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083), expandable thumbnail

    OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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