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AB193494

Anti-Pertussis toxin antibody

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(1 Publication)

Rabbit Polyclonal TOX1 antibody. Suitable for ELISA, WB and reacts with Bordetella pertussis samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Bordetella pertussis Tohama I ptxA.

View Alternative Names

BP3783, ptxA, Pertussis toxin subunit 1, PTX S1, Islet-activating protein S1, NAD-dependent ADP-ribosyltransferase, IAP S1

1 Images
Western blot - Anti-Pertussis toxin antibody (AB193494)
  • WB

Supplier Data

Western blot - Anti-Pertussis toxin antibody (AB193494)

All lanes:

Western blot - Anti-Pertussis toxin antibody (ab193494) at 2 µg/mL

All lanes:

Recombinant Bordella pertussis pertussis toxin subunit 1 at 0.1 µg

Secondary

All lanes:

Polyclonal goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Bordetella pertussis

Applications

WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Bordetella pertussis Tohama I ptxA.

P04977

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pertussis toxin also known as PT or PTx is a protein with an approximate mass of 105-115 kDa. The bacterium Bordetella pertussis expresses this toxin which consists of five subunits arranged in an A-B toxin structure. The A portion functions as the enzymatic domain while the B units facilitate binding to host cell receptors. Pertussis toxin executes its mechanical function by catalyzing the ADP-ribosylation of the Gi protein which inhibits signal transduction pathways by immobilizing the G-protein causing disruption in normal cellular communication.
Biological function summary

This toxin disrupts immune surveillance and modulates host immune responses to facilitate infection by Bordetella pertussis. Pertussis toxin is not an isolated agent but functions in concert with other virulence factors produced by the bacterium. This synergy potentiates its ability to impair neutrophil recruitment and function. Moreover PT impacts the ciliary activity in the respiratory tract contributing to the characteristic coughing in whooping cough by limiting the clearance of respiratory secretions.

Pathways

ADP-ribosylation by pertussis toxin directly affects the inhibitory G-proteins specifically Giα which play important roles in several signaling pathways including the cAMP signaling pathway. The toxin's ability to increase the levels of cAMP disrupts normal intracellular signaling leading to altered leukocyte behavior. Such disruptions extend its influence into pathways involving proteins such as adenylyl cyclase and phospholipase C which further modulate immune responses.

The pertussis toxin is intrinsically linked to whooping cough caused by Bordetella pertussis infection. This toxin contributes to the severe coughing fits and systemic symptoms characteristic of the disease. Additionally pertussis toxin's immune-modulating properties also show relevance in the context of autoimmune disorders as it may impact proteins involved in immune tolerance such as the aforementioned G-proteins shaping the body's response to self-antigens. Understanding pertussis toxin and its related pathways can guide the development of vaccines and therapeutic antibodies targeting pertussis infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.
See full target information ptxA

Additional targets

Pertussis toxin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 41:3327-3341 PubMed29512687

2018

Transcriptomic and proteomic analysis of potential therapeutic target genes in the liver of metformin‑treated Sprague‑Dawley rats with type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Yitao Chen,Yangsheng Wu,Yuanxiao Yang,Zhiwei Xu,Junfeng Tong,Zheming Li,Xiaojie Zhou,Changyu Li
View all publications
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