AB252849

Anti-Pescadillo antibody [8E9]

Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rat Recombinant Monoclonal Pescadillo antibody. Suitable for WB, IF and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

Pescadillo homolog, PES1

3 Images

Supplier Data

Immunofluorescence - Anti-Pescadillo antibody [8E9] (AB252849)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293 (human embryonic kidney epithelial cell) labeling Pescadillo with ab252849 at 0.978μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HEK-293 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :

-ve control 1 : ab252849 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.

-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

Supplier Data

Immunofluorescence - Anti-Pescadillo antibody [8E9] (AB252849)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling Pescadillo with ab252849 at 0.978μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

The negative controls are as follows :

-ve control 1 : ab252849 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.

-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

Supplier Data

Western blot - Anti-Pescadillo antibody [8E9] (AB252849)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-4 : 1 sec; Lane 5 : 5 secs.

All lanes:

Western blot - Anti-Pescadillo antibody [8E9] (ab252849) at 0.489 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/100000 dilution

Predicted band size: 68 kDa

false

Carrier free

Anti-Pescadillo antibody [8E9] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

8E9

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.489 µg/mL", "WB-species-notes": "", "IF-species-checked": "testedAndGuaranteed", "IF-species-dilution-info": "0.978 µg/mL", "IF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.489 µg/mL", "WB-species-notes": "", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pescadillo also known as Pes1 is a nucleolar protein with an approximate molecular mass of 110 kDa. It is expressed in the nucleolus of human cells and plays a significant mechanical role in ribosome biogenesis. The protein functions in the processing of the 47S pre-rRNA transcript into 18S and 28S rRNAs which are essential components of the small and large ribosomal subunits respectively. Pescadillo is also involved in cell proliferation indicated by its higher expression levels in rapidly dividing cells such as cancer cells.

Biological function summary

The function of Pescadillo extends beyond ribosome production. It forms a part of a larger complex involved in the maturation of pre-ribosomal RNA interacting with proteins like BOP1 and WDR12. This interaction signifies its role in the PeBoW complex important for proper cell division and growth. Pescadillo influences the cell cycle and has implications in regulating p53-dependent pathways which are critical for maintaining genomic stability.

Pathways

Pescadillo plays a part in the ribosome biogenesis pathway and p53 signaling pathway. In ribosome biogenesis it works alongside other proteins such as NPM1 and RPL5 to ensure accurate ribosome assembly necessary for protein synthesis. Within the p53 signaling pathway Pescadillo modulates cell cycle checkpoints preventing DNA damage from leading to cancerous growths through its modulation of p53 an important tumor suppressor.

Pescadillo's aberrant function or expression links to cancer and developmental disorders. Its overexpression commonly associates with breast cancer where it may serve as a biomarker due to its involvement in increased cell proliferation. Additionally mutations in genes regulating Pescadillo activity can lead to disorders in embryonic development as protein partners like RPL5 are implicated in these processes. Understanding its regulatory mechanisms offers potential therapeutic targets in oncology and developmental biology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information PES1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7799 PubMed40921755

2025

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Matsuhashi,Kei K Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S Stewart,Shoji Hata,Daiju Kitagawa

Cancer medicine 12:5703-5717 PubMed36217758

2022

Pescadillo ribosomal biogenesis factor 1 reduction suppresses tumour growth and renders chemosensitivity of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Li,Changyu Yao,Zhao Ding,Ping Liu,Xue Chen,Weiwei Liu,Fangzheng Yi,Chuanya Jiang,Hongwu Li,Yehai Liu,Jing Wu

eLife 11: PubMed35674491

2022

METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Eriko Matsuura-Suzuki,Tadahiro Shimazu,Mari Takahashi,Kaoru Kotoshiba,Takehiro Suzuki,Kazuhiro Kashiwagi,Yoshihiro Sohtome,Mai Akakabe,Mikiko Sodeoka,Naoshi Dohmae,Takuhiro Ito,Yoichi Shinkai,Shintaro Iwasaki

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pescadillo antibody [8E9]

Immunofluorescence - Anti-Pescadillo antibody [8E9] (AB252849)

Immunofluorescence - Anti-Pescadillo antibody [8E9] (AB252849)

Western blot - Anti-Pescadillo antibody [8E9] (AB252849)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise