AB72539

Anti-Pescadillo antibody

Name: Anti-Pescadillo antibody (ab72539) | Abcam
Brand: Abcam Limited
SKU: AB72539

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(5 Publications)

Rabbit Polyclonal Pescadillo antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PES1.

View Alternative Names

Pescadillo homolog, PES1

2 Images

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Immunoprecipitation - Anti-Pescadillo antibody (AB72539)

Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

All lanes:

Immunoprecipitation - Anti-Pescadillo antibody (ab72539)

Predicted band size: 68 kDa

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Western blot - Anti-Pescadillo antibody (AB72539)

All lanes:

Western blot - Anti-Pescadillo antibody (ab72539) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK 293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PES1. The exact immunogen used to generate this antibody is proprietary information.

O00541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pescadillo also known as Pes1 is a nucleolar protein with an approximate molecular mass of 110 kDa. It is expressed in the nucleolus of human cells and plays a significant mechanical role in ribosome biogenesis. The protein functions in the processing of the 47S pre-rRNA transcript into 18S and 28S rRNAs which are essential components of the small and large ribosomal subunits respectively. Pescadillo is also involved in cell proliferation indicated by its higher expression levels in rapidly dividing cells such as cancer cells.

Biological function summary

The function of Pescadillo extends beyond ribosome production. It forms a part of a larger complex involved in the maturation of pre-ribosomal RNA interacting with proteins like BOP1 and WDR12. This interaction signifies its role in the PeBoW complex important for proper cell division and growth. Pescadillo influences the cell cycle and has implications in regulating p53-dependent pathways which are critical for maintaining genomic stability.

Pathways

Pescadillo plays a part in the ribosome biogenesis pathway and p53 signaling pathway. In ribosome biogenesis it works alongside other proteins such as NPM1 and RPL5 to ensure accurate ribosome assembly necessary for protein synthesis. Within the p53 signaling pathway Pescadillo modulates cell cycle checkpoints preventing DNA damage from leading to cancerous growths through its modulation of p53 an important tumor suppressor.

Pescadillo's aberrant function or expression links to cancer and developmental disorders. Its overexpression commonly associates with breast cancer where it may serve as a biomarker due to its involvement in increased cell proliferation. Additionally mutations in genes regulating Pescadillo activity can lead to disorders in embryonic development as protein partners like RPL5 are implicated in these processes. Understanding its regulatory mechanisms offers potential therapeutic targets in oncology and developmental biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information PES1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 37:564-573 PubMed34845814

2021

Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Dong Xu,Yi Miao,Pan Li,Ping-Ping Li,Jie Liu,Juan Li,Fang Cao

Journal of translational medicine 18:209 PubMed32448371

2020

microRNA-1271 impedes the development of prostate cancer by downregulating PES1 and upregulating ERβ.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenming Jiang,Yuxi Zhang,Xi Chen,Yan Wang,Pingeng Wu,Chengzhang Wu,Dong Chen

Journal of experimental & clinical cancer research 38:463 PubMed31718704

2019

PES1 promotes BET inhibitors resistance and cells proliferation through increasing c-Myc expression in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jin,Rui Fang,Ping Fan,Lipeng Zeng,Bin Zhang,Xiaoming Lu,Tao Liu

Life sciences 231:116571 PubMed31207308

2019

Linear and circular CDKN2B-AS1 expression is associated with Inflammatory Bowel Disease and participates in intestinal barrier formation.

Applications

Unspecified application

Species

Unspecified reactive species

Carl Robert Rankin,Zulfiqar Ali Lokhandwala,Raymond Huang,Joel Pekow,Charalabos Pothoulakis,David Padua

Scientific reports 9:1032 PubMed30705367

2019

PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERα/ERβ protein ratio.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Bo Qiu,Ling-Yao Liao,Rong Jiang,Man Xu,Lin-Wan Xu,George G Chen,Zhi-Min Liu

View all publications

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Product promise

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Anti-Pescadillo antibody

Immunoprecipitation - Anti-Pescadillo antibody (AB72539)

Western blot - Anti-Pescadillo antibody (AB72539)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise