AB88543

Anti-Pescadillo antibody

Name: Anti-Pescadillo antibody (ab88543) | Abcam
Brand: Abcam Limited
SKU: AB88543
Price: 545 USD
Availability: InStock

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(3 Publications)

Mouse Polyclonal Pescadillo antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PES1.

View Alternative Names

Pescadillo homolog, PES1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Pescadillo antibody (AB88543)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Pescadillo antibody (AB88543)

ab88543, at 10 μg/ml, staining Pescadillo in HeLa cell by Immunofluorescence.

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Western blot - Anti-Pescadillo antibody (AB88543)

All lanes:

Western blot - Anti-Pescadillo antibody (ab88543) at 1 µg/mL

All lanes:

A-431 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 68 kDa

Observed band size: 73 kDa

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Western blot - Anti-Pescadillo antibody (AB88543)

All lanes:

Western blot - Anti-Pescadillo antibody (ab88543) at 1 µg/mL

All lanes:

PC-12 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Unknown

Western blot - Anti-Pescadillo antibody (AB88543)

All lanes:

Western blot - Anti-Pescadillo antibody (ab88543) at 1 µg/mL

Lane 1:

Pescadillo transfected 293T cell lysate at 50 µg

Lane 2:

Non transfected 293T cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 68 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PES1.

O00541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pescadillo also known as Pes1 is a nucleolar protein with an approximate molecular mass of 110 kDa. It is expressed in the nucleolus of human cells and plays a significant mechanical role in ribosome biogenesis. The protein functions in the processing of the 47S pre-rRNA transcript into 18S and 28S rRNAs which are essential components of the small and large ribosomal subunits respectively. Pescadillo is also involved in cell proliferation indicated by its higher expression levels in rapidly dividing cells such as cancer cells.

Biological function summary

The function of Pescadillo extends beyond ribosome production. It forms a part of a larger complex involved in the maturation of pre-ribosomal RNA interacting with proteins like BOP1 and WDR12. This interaction signifies its role in the PeBoW complex important for proper cell division and growth. Pescadillo influences the cell cycle and has implications in regulating p53-dependent pathways which are critical for maintaining genomic stability.

Pathways

Pescadillo plays a part in the ribosome biogenesis pathway and p53 signaling pathway. In ribosome biogenesis it works alongside other proteins such as NPM1 and RPL5 to ensure accurate ribosome assembly necessary for protein synthesis. Within the p53 signaling pathway Pescadillo modulates cell cycle checkpoints preventing DNA damage from leading to cancerous growths through its modulation of p53 an important tumor suppressor.

Pescadillo's aberrant function or expression links to cancer and developmental disorders. Its overexpression commonly associates with breast cancer where it may serve as a biomarker due to its involvement in increased cell proliferation. Additionally mutations in genes regulating Pescadillo activity can lead to disorders in embryonic development as protein partners like RPL5 are implicated in these processes. Understanding its regulatory mechanisms offers potential therapeutic targets in oncology and developmental biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information PES1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 37:564-573 PubMed34845814

2021

Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Dong Xu,Yi Miao,Pan Li,Ping-Ping Li,Jie Liu,Juan Li,Fang Cao

Gastroenterology : PubMed32805281

2020

The Long Noncoding RNA CCAT2 induces chromosomal instability through BOP1 - AURKB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Baoqing Chen,Mihnea P Dragomir,Linda Fabris,Recep Bayraktar,Erik Knutsen,Xu Liu,Changyan Tang,Yongfeng Li,Tadanobu Shimura,Tina Catela Ivkovic,Mireia Cruz De Los Santos,Simone Anfossi,Masayoshi Shimizu,Maitri Y Shah,Hui Ling,Peng Shen,Asha S Multani,Barbara Pardini,Jared K Burks,Hiroyuki Katayama,Lucas C Reineke,Longfei Huo,Muddassir Syed,Shumei Song,Manuela Ferracin,Eiji Oki,Bastian Fromm,Cristina Ivan,Krithika Bhuvaneshwar,Yuriy Gusev,Koshi Mimori,David Menter,Subrata Sen,Takatoshi Matsuyama,Hiroyuki Uetake,Catalin Vasilescu,Scott Kopetz,Jan Parker-Thornburg,Ayumu Taguchi,Samir M Hanash,Leonard Girnita,Ondrej Slaby,Ajay Goel,Gabriele Varani,Mihai Gagea,Chunlai Li,Jaffer A Ajani,George A Calin

Cancer science 106:237-43 PubMed25557119

2015

Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation.

Applications

ICC

Species

Unspecified reactive species

Masato Nakaguro,Shinichi Kiyonari,Satoshi Kishida,Dongliang Cao,Yuko Murakami-Tonami,Hitoshi Ichikawa,Ichiro Takeuchi,Shigeo Nakamura,Kenji Kadomatsu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pescadillo antibody

Immunocytochemistry/ Immunofluorescence - Anti-Pescadillo antibody (AB88543)

Western blot - Anti-Pescadillo antibody (AB88543)

Western blot - Anti-Pescadillo antibody (AB88543)

Western blot - Anti-Pescadillo antibody (AB88543)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise