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Rabbit Monoclonal FEV antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FEV.

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Images

Western blot - Anti-Pet1 antibody [HL2043] (AB308220), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human FEV. Database link Q99581

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.

Alternative names

PET1, FEV, Protein FEV, Fifth Ewing variant protein, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, Pet-1

Recommended products

Rabbit Monoclonal FEV antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FEV.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human FEV. Database link Q99581
Carrier free
Yes
Clone number
HL2043
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Pet1 protein also known as FEVER or Fev is a transcription factor with a mass of approximately 27 kDa. It is a member of the ETS (E26 transformation-specific) family and is principally expressed in serotonergic neurons of the raphe nuclei within the brain. This expression pattern is consistent with its role in the development and maintenance of specific neuronal subtypes.

Biological function summary

Pet1 drives the differentiation development and function of serotonergic neurons. It acts as a critical regulator by binding to DNA sequences to control the transcription of genes involved in neurotransmitter synthesis and transport. Pet1 forms complexes with other transcription factors enhancing its regulatory actions and it is especially important for the production of the neurotransmitter serotonin.

Pathways

Pet1 holds a significant role in the serotonin biosynthesis pathway. It regulates genes such as tryptophan hydroxylase 2 (TPH2) and the serotonin transporter (SERT) which are necessary for serotonin metabolism and its transport. Pet1 interacts with other proteins like GATA3 to fine-tune the serotonergic lineage specification and functions in a network that impacts mood regulation.

Associated diseases and disorders

Aberrations in Pet1 expression or function have links to conditions like depression and anxiety disorders. The dysfunction of serotonergic neurons in which Pet1 plays an important role contributes to these mental health disorders. Moreover alterations in Pet1 can influence the expression of receptor protein 5-HT1A which has implications in both the onset and treatment responses of depressive disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Pet1 antibody [HL2043] (ab308220), expandable thumbnail

    Western blot - Anti-Pet1 antibody [HL2043] (ab308220)

    SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pet1 antibody [HL2043] (ab308220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: SH-SY5Y whole cell extract at 30 µg

    Lane 2: SH-SY5Y nuclear extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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