Rabbit Polyclonal PEX1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PEX1 aa 1200 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PEX1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43933
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the PEX1-PEX6 AAA ATPase complex, a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling (PubMed:11439091, PubMed:16314507, PubMed:16854980, PubMed:21362118, PubMed:29884772). Specifically recognizes PEX5 monoubiquitinated at 'Cys-11', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel (PubMed:29884772). Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5 (PubMed:29884772).
Alternative names
Peroxisomal ATPase PEX1, Peroxin-1, Peroxisome biogenesis disorder protein 1, Peroxisome biogenesis factor 1, PEX1
Recommended products
Rabbit Polyclonal PEX1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PEX1 aa 1200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PEX1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43933
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PEX1 also known as Peroxisomal Biogenesis Factor 1 plays an important role in peroxisomal function. PEX1 has a molecular mass of around 145 kDa and is widely expressed in human tissues. It belongs to the family of ATPases associated with various cellular activities (AAAs) which are essential molecular machines involved in diverse biological processes. PEX1 localizes mainly to the peroxisomes which means it takes part in the mechanics of peroxisome assembly and function.
- Biological function summary
PEX1 interacts in collaboration with other peroxisomal proteins to facilitate the import of matrix proteins into the peroxisome. It exists as part of a hetero-oligomeric complex with PEX6 another AAA protein which forms an important part of the peroxisome biogenesis mechanism. PEX1’s function in this complex contributes to the assembly maintenance and selective import operations within the peroxisome which are critical for cellular homeostasis and lipid metabolism.
- Pathways
PEX1 contributes significantly within the peroxisomal biogenesis pathway where it works alongside proteins like PEX2 and PEX6 to maintain the functionality of the peroxisomes. This pathway is pivotal in multiple biological functions including plasmalogen synthesis and the breakdown of very-long-chain fatty acids. These processes tie closely with broader cellular metabolic pathways impacting both energy regulation and lipid processing.
- Associated diseases and disorders
Defects in PEX1 are linked to peroxisome biogenesis disorders such as Zellweger syndrome spectrum. These disorders result from compromised peroxisomal function leading to severe clinical symptoms and often fatal outcomes. PEX1 mutations disrupt its interaction with PEX6 impairing peroxisome function. This highlights its important role in peroxisome-related diseases and provides potential targets for future therapeutic strategies to treat these metabolic conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-PEX1 antibody (ab217059)
Detection of PEX1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217059 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab217059 was used at 1μg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-PEX1 antibody (ab217059)
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3min
Western blot - Anti-PEX1 antibody (ab217059)
All lanes: Western blot - Anti-PEX1 antibody (ab217059) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: mouse TCMK1 whole cell lysate at 50 µg
Lane 5: mouse NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3min
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