AB217059

Anti-PEX1 antibody

Name: Anti-PEX1 antibody (ab217059) | Abcam
Brand: Abcam Limited
SKU: AB217059
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PEX1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PEX1 aa 1200 to C-terminus.

View Alternative Names

Peroxisomal ATPase PEX1, Peroxin-1, Peroxisome biogenesis disorder protein 1, Peroxisome biogenesis factor 1, PEX1

2 Images

Supplier Data

Immunoprecipitation - Anti-PEX1 antibody (AB217059)

Detection of PEX1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217059 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab217059 was used at 1μg/ml. Lane 2 Control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PEX1 antibody (ab217059)

Predicted band size: 142 kDa

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Exposure time: 3min

Supplier Data

Western blot - Anti-PEX1 antibody (AB217059)

All lanes:

Western blot - Anti-PEX1 antibody (ab217059) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

mouse TCMK1 whole cell lysate at 50 µg

Lane 5:

mouse NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 142 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PEX1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43933

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PEX1 also known as Peroxisomal Biogenesis Factor 1 plays an important role in peroxisomal function. PEX1 has a molecular mass of around 145 kDa and is widely expressed in human tissues. It belongs to the family of ATPases associated with various cellular activities (AAAs) which are essential molecular machines involved in diverse biological processes. PEX1 localizes mainly to the peroxisomes which means it takes part in the mechanics of peroxisome assembly and function.

Biological function summary

PEX1 interacts in collaboration with other peroxisomal proteins to facilitate the import of matrix proteins into the peroxisome. It exists as part of a hetero-oligomeric complex with PEX6 another AAA protein which forms an important part of the peroxisome biogenesis mechanism. PEX1’s function in this complex contributes to the assembly maintenance and selective import operations within the peroxisome which are critical for cellular homeostasis and lipid metabolism.

Pathways

PEX1 contributes significantly within the peroxisomal biogenesis pathway where it works alongside proteins like PEX2 and PEX6 to maintain the functionality of the peroxisomes. This pathway is pivotal in multiple biological functions including plasmalogen synthesis and the breakdown of very-long-chain fatty acids. These processes tie closely with broader cellular metabolic pathways impacting both energy regulation and lipid processing.

Defects in PEX1 are linked to peroxisome biogenesis disorders such as Zellweger syndrome spectrum. These disorders result from compromised peroxisomal function leading to severe clinical symptoms and often fatal outcomes. PEX1 mutations disrupt its interaction with PEX6 impairing peroxisome function. This highlights its important role in peroxisome-related diseases and provides potential targets for future therapeutic strategies to treat these metabolic conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the PEX1-PEX6 AAA ATPase complex, a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling (PubMed : 11439091, PubMed : 16314507, PubMed : 16854980, PubMed : 21362118, PubMed : 29884772). Specifically recognizes PEX5 monoubiquitinated at 'Cys-11', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel (PubMed : 29884772). Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5 (PubMed : 29884772).

See full target information PEX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1602 PubMed36959177

2023

Mena regulates nesprin-2 to control actin-nuclear lamina associations, trans-nuclear membrane signalling and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Frederic Li Mow Chee,Bruno Beernaert,Billie G C Griffith,Alexander E P Loftus,Yatendra Kumar,Jimi C Wills,Martin Lee,Jessica Valli,Ann P Wheeler,J Douglas Armstrong,Maddy Parsons,Irene M Leigh,Charlotte M Proby,Alex von Kriegsheim,Wendy A Bickmore,Margaret C Frame,Adam Byron

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEX1 antibody

Immunoprecipitation - Anti-PEX1 antibody (AB217059)

Western blot - Anti-PEX1 antibody (AB217059)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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