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AB250321

Anti-PEX11B antibody [EPR12183] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PEX11B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Peroxisomal membrane protein 11B, Peroxin-11B, Peroxisomal biogenesis factor 11B, Protein PEX11 homolog beta, PEX11-beta, PEX11B

2 Images
Western blot - Anti-PEX11B antibody [EPR12183] - BSA and Azide free (AB250321)
  • WB

Supplier Data

Western blot - Anti-PEX11B antibody [EPR12183] - BSA and Azide free (AB250321)

This data was developed using ab181066, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PEX11B antibody [EPR12183] (<a href='/en-us/products/primary-antibodies/pex11b-antibody-epr12183-ab181066'>ab181066</a>) at 1/10000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-PEX11B antibody [EPR12183] - BSA and Azide free (AB250321)
  • WB

Supplier Data

Western blot - Anti-PEX11B antibody [EPR12183] - BSA and Azide free (AB250321)

This data was developed using ab181066, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PEX11B antibody [EPR12183] (<a href='/en-us/products/primary-antibodies/pex11b-antibody-epr12183-ab181066'>ab181066</a>) at 1/10000 dilution

Lane 1:

HUVEC cell lysate at 20 µg

Lane 2:

HT-29 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12183

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250321 is the carrier-free version of ab181066.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX11B or peroxisomal biogenesis factor 11 beta is a protein involved in the maintenance and proliferation of peroxisomes. The protein has a molecular mass of approximately 27 kDa. PEX11B is expressed throughout different tissues but has higher expression levels in tissues with high metabolic activity such as the liver brain and kidneys. This protein belongs to the PEX family which plays a role in peroxisome dynamics.
Biological function summary

PEX11B participates in the elongation and division of peroxisomes. It does not function independently but instead interacts with other peroxisomal biogenesis factors as part of a larger complex responsible for peroxisome morphogenesis. This action is important for maintaining proper peroxisome numbers and adapting cell compartmentalization according to metabolic demands. By affecting peroxisome dynamics PEX11B indirectly influences cellular processes like fatty acid beta-oxidation and reactive oxygen species detoxification.

Pathways

PEX11B's role is significant in the peroxisomal biogenesis pathway facilitating peroxisome multiplication. It connects with other proteins such as DRP1 which assists in membrane fission during division. The coordinated function of these proteins ensures proper peroxisomal turnover and function. Additionally PEX11B influences the cellular response to oxidative stress by regulating peroxisome numbers which in turn impacts cellular signaling pathways involved in stress response.

PEX11B mutations are linked to certain peroxisome biogenesis disorders like Zellweger spectrum disorders. These mutations can lead to impaired peroxisome function resulting in critical metabolic imbalances and developmental issues. PEX11B closely associates with other peroxin proteins such as PEX1 and PEX6 which share roles in peroxisomal assembly. Together they highlight the impact of peroxisome homeostasis on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in peroxisomal proliferation (PubMed : 9792670). May regulate peroxisome division by recruiting the dynamin-related GTPase DNM1L to the peroxisomal membrane (PubMed : 12618434). Promotes membrane protrusion and elongation on the peroxisomal surface (PubMed : 20826455).
See full target information PEX11B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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