Rabbit Polyclonal PEX14 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PEX14.
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Publications
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- Cell death & disease 9:6572018S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Xia et. al.PubMed 29844306
- The Journal of cell biology 216:2843-28582017TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Zhi-Jie Xia,Jean-Claude Farré,Suresh SubramaniPubMed 28724525
- Nature cell biology 17:1259-12692015ATM functions at the peroxisome to induce pexophagy in response to ROS.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PEX14. The exact immunogen used to generate this antibody is proprietary information. Database link O75381
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed:24235149, PubMed:28765278, PubMed:9653144). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed:24235149, PubMed:28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed:21525035).
Alternative names
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14
Recommended products
Rabbit Polyclonal PEX14 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PEX14.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PEX14. The exact immunogen used to generate this antibody is proprietary information. Database link O75381
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEX14 also known as Peroxisomal Membrane Protein 14 is an essential component of the peroxisomal membrane acting as an integral membrane protein. PEX14 has a mass of approximately 47 kDa and is expressed in many tissues including liver and kidney. It functions mechanically as a docking factor within the peroxisomal matrix enabling the import of proteins into peroxisomes which are cellular organelles responsible for various metabolic functions.
- Biological function summary
PEX14 plays a significant role in maintaining peroxisome structure and function. It is part of the peroxisome importomer complex which facilitates the import of matrix proteins by interacting with peroxins such as PEX5. PEX14's interaction with these proteins ensures the proper translocation and sorting of enzymes critical for peroxisomal activities like fatty acid oxidation and reactive oxygen species detoxification.
- Pathways
PEX14 is involved in key metabolic and cellular pathways including peroxisomal fatty acid beta-oxidation and ether lipid metabolism. It directly associates with PEX5 a peroxin that transports cargo proteins. In these pathways PEX14 plays a central role in maintaining metabolic processes that are essential for lipid metabolism and cellular detoxification.
- Associated diseases and disorders
PEX14’s malfunction or absence can contribute to Peroxisome Biogenesis Disorders (PBDs) such as Zellweger syndrome. These disorders significantly affect cellular metabolism and result in severe developmental issues. PEX14's interactions with proteins like PEX19 affect peroxisomal membrane biogenesis further linking PEX14 to these metabolic disorders and highlighting its importance in peroxisomal function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (ab109999)
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab109999.
Western blot - Anti-PEX14 antibody (ab109999)
All lanes: Western blot - Anti-PEX14 antibody (ab109999) at 1/500 dilution
Lane 1: A549 cells at 5 µg
Lane 2: A549 cells treated with the synthesized peptide at 5 µg
Predicted band size: 15 kDa, 23 kDa, 41 kDa, 59 kDa, 73 kDa
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