AB109999

Anti-PEX14 antibody

Name: Anti-PEX14 antibody (ab109999) | Abcam
Brand: Abcam Limited
SKU: AB109999
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PEX14 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PEX14.

View Alternative Names

Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab109999.

Unknown

Western blot - Anti-PEX14 antibody (AB109999)

All lanes:

Western blot - Anti-PEX14 antibody (ab109999) at 1/500 dilution

Lane 1:

A549 cells at 5 µg

Lane 2:

A549 cells treated with the synthesized peptide at 5 µg

Predicted band size: 15 kDa,23 kDa,41 kDa,59 kDa,73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PEX14. The exact immunogen used to generate this antibody is proprietary information.

O75381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX14 also known as Peroxisomal Membrane Protein 14 is an essential component of the peroxisomal membrane acting as an integral membrane protein. PEX14 has a mass of approximately 47 kDa and is expressed in many tissues including liver and kidney. It functions mechanically as a docking factor within the peroxisomal matrix enabling the import of proteins into peroxisomes which are cellular organelles responsible for various metabolic functions.

Biological function summary

PEX14 plays a significant role in maintaining peroxisome structure and function. It is part of the peroxisome importomer complex which facilitates the import of matrix proteins by interacting with peroxins such as PEX5. PEX14's interaction with these proteins ensures the proper translocation and sorting of enzymes critical for peroxisomal activities like fatty acid oxidation and reactive oxygen species detoxification.

Pathways

PEX14 is involved in key metabolic and cellular pathways including peroxisomal fatty acid beta-oxidation and ether lipid metabolism. It directly associates with PEX5 a peroxin that transports cargo proteins. In these pathways PEX14 plays a central role in maintaining metabolic processes that are essential for lipid metabolism and cellular detoxification.

PEX14’s malfunction or absence can contribute to Peroxisome Biogenesis Disorders (PBDs) such as Zellweger syndrome. These disorders significantly affect cellular metabolism and result in severe developmental issues. PEX14's interactions with proteins like PEX19 affect peroxisomal membrane biogenesis further linking PEX14 to these metabolic disorders and highlighting its importance in peroxisomal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed : 24235149, PubMed : 28765278, PubMed : 9653144). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed : 24235149, PubMed : 28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed : 21525035).

See full target information PEX14

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 9:657 PubMed29844306

2018

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Xing Li,Huijuan Zhou,Lu Zheng,Jing Shi

The Journal of cell biology 216:2843-2858 PubMed28724525

2017

TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Zhi-Jie Xia,Jean-Claude Farré,Suresh Subramani

Nature cell biology 17:1259-1269 PubMed26344566

2015

ATM functions at the peroxisome to induce pexophagy in response to ROS.

Applications

Species

Human

Jiangwei Zhang,Durga Nand Tripathi,Ji Jing,Angela Alexander,Jinhee Kim,Reid T Powell,Ruhee Dere,Jacqueline Tait-Mulder,Ji-Hoon Lee,Tanya T Paull,Raj K Pandita,Vijaya K Charaka,Tej K Pandita,Michael B Kastan,Cheryl Lyn Walker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEX14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

Western blot - Anti-PEX14 antibody (AB109999)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise