AB183885

Anti-PEX14 antibody

Name: Anti-PEX14 antibody (ab183885) | Abcam
Brand: Abcam Limited
SKU: AB183885
Price: 505 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal PEX14 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus.

View Alternative Names

Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (AB183885)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (AB183885)

Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells labeling PEX14 with ab183885 at 1/500 dilution (green) and Hoechst 33342 (blue). Cells were fixed in 4% paraformaldehyde at room temperature for 15 mins.

Supplier Data

Western blot - Anti-PEX14 antibody (AB183885)

10% SDS PAGE

All lanes:

Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-PEX14 antibody (AB183885)

10% SDS-PAGE

All lanes:

Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-PEX14 antibody (AB183885)

10% SDS-PAGE

All lanes:

Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX14 also known as Peroxisomal Membrane Protein 14 is an essential component of the peroxisomal membrane acting as an integral membrane protein. PEX14 has a mass of approximately 47 kDa and is expressed in many tissues including liver and kidney. It functions mechanically as a docking factor within the peroxisomal matrix enabling the import of proteins into peroxisomes which are cellular organelles responsible for various metabolic functions.

Biological function summary

PEX14 plays a significant role in maintaining peroxisome structure and function. It is part of the peroxisome importomer complex which facilitates the import of matrix proteins by interacting with peroxins such as PEX5. PEX14's interaction with these proteins ensures the proper translocation and sorting of enzymes critical for peroxisomal activities like fatty acid oxidation and reactive oxygen species detoxification.

Pathways

PEX14 is involved in key metabolic and cellular pathways including peroxisomal fatty acid beta-oxidation and ether lipid metabolism. It directly associates with PEX5 a peroxin that transports cargo proteins. In these pathways PEX14 plays a central role in maintaining metabolic processes that are essential for lipid metabolism and cellular detoxification.

PEX14’s malfunction or absence can contribute to Peroxisome Biogenesis Disorders (PBDs) such as Zellweger syndrome. These disorders significantly affect cellular metabolism and result in severe developmental issues. PEX14's interactions with proteins like PEX19 affect peroxisomal membrane biogenesis further linking PEX14 to these metabolic disorders and highlighting its importance in peroxisomal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed : 24235149, PubMed : 28765278, PubMed : 9653144). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed : 24235149, PubMed : 28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed : 21525035).

See full target information PEX14

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 98:e0109924 PubMed39480111

2024

HCMV strain- and cell type-specific alterations in membrane contact sites point to the convergent regulation of organelle remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

William A Hofstadter,Ji Woo Park,Krystal K Lum,Sophia Chen,Ileana M Cristea

mBio 15:e0035324 PubMed38984828

2024

Physiologic medium renders human iPSC-derived macrophages permissive for by rewiring organelle function and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Bussi,Rachel Lai,Natalia Athanasiadi,Maximiliano G Gutierrez

Cell communication and signaling : CCS 22:142 PubMed38383392

2024

TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Laxman Manandhar,Raghbendra Kumar Dutta,Pradeep Devkota,Arun Chhetri,Xiaofan Wei,Channy Park,Hyug Moo Kwon,Raekil Park

Cell reports methods 4:100692 PubMed38232737

2024

Using MitER for 3D analysis of mitochondrial morphology and ER contacts.

Applications

Unspecified application

Species

Unspecified reactive species

Therese Kichuk,Satyen Dhamankar,Saurabh Malani,William A Hofstadter,Scott A Wegner,Ileana M Cristea,José L Avalos

The Journal of cell biology 222: PubMed37737955

2023

Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G Gutierrez

Nature communications 13:4720 PubMed35953480

2022

Restructured membrane contacts rewire organelles for human cytomegalovirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Katelyn C Cook,Elene Tsopurashvili,Jason M Needham,Sunnie R Thompson,Ileana M Cristea

Scientific reports 11:10133 PubMed33980926

2021

A human infertility-associated KASH5 variant promotes mitochondrial localization.

Applications

Unspecified application

Species

Unspecified reactive species

Sana A Bentebbal,Bakhita R Meqbel,Anna Salter,Victoria Allan,Brian Burke,Henning F Horn

Cell reports 32:107943 PubMed32726614

2020

Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA.

Applications

Unspecified application

Species

Unspecified reactive species

Joel D Federspiel,Katelyn C Cook,Michelle A Kennedy,Samvida S Venkatesh,Clayton J Otter,William A Hofstadter,Pierre M Jean Beltran,Ileana M Cristea

The EMBO journal 38:e101994 PubMed31625181

2019

Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs.

Applications

Unspecified application

Species

Unspecified reactive species

Yuexi Gu,Yakubu Princely Abudu,Suresh Kumar,Bhawana Bissa,Seong Won Choi,Jingyue Jia,Michael Lazarou,Eeva-Liisa Eskelinen,Terje Johansen,Vojo Deretic

Cell host & microbe 24:526-541.e7 PubMed30269970

2018

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre M Jean Beltran,Katelyn C Cook,Yutaka Hashimoto,Cyril Galitzine,Laura A Murray,Olga Vitek,Ileana M Cristea

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEX14 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (AB183885)

Western blot - Anti-PEX14 antibody (AB183885)

Western blot - Anti-PEX14 antibody (AB183885)

Western blot - Anti-PEX14 antibody (AB183885)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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