Rabbit Polyclonal PEX14 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus.
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Publications
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- The Journal of cell biology 222:2023Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G GutierrezPubMed 37737955
- Nature communications 13:47202022Restructured membrane contacts rewire organelles for human cytomegalovirus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatelyn C Cook,Elene Tsopurashvili,Jason M Needham,Sunnie R Thompson,Ileana M CristeaPubMed 35953480
- Scientific reports 11:101332021A human infertility-associated KASH5 variant promotes mitochondrial localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSana A Bentebbal,Bakhita R Meqbel,Anna Salter,Victoria Allan,Brian Burke,Henning F HornPubMed 33980926
- Cell reports 32:1079432020Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoel D Federspiel,Katelyn C Cook,Michelle A Kennedy,Samvida S Venkatesh,Clayton J Otter,William A Hofstadter,Pierre M Jean Beltran,Ileana M CristeaPubMed 32726614
- The EMBO journal 38:e1019942019Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuexi Gu et. al.PubMed 31625181
- Cell host & microbe 24:526-541.e72018Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPierre M Jean Beltran et. al.PubMed 30269970
- Current protocols in cell biology 62:21.9.1-21.9.202014Probing peroxisome dynamics and biogenesis by fluorescence imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiluska Jauregui,Peter K KimPubMed 24610124
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75381
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed:24235149, PubMed:28765278, PubMed:9653144). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed:24235149, PubMed:28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed:21525035).
Alternative names
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14
Recommended products
Rabbit Polyclonal PEX14 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PEX14 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75381
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEX14 also known as Peroxisomal Membrane Protein 14 is an essential component of the peroxisomal membrane acting as an integral membrane protein. PEX14 has a mass of approximately 47 kDa and is expressed in many tissues including liver and kidney. It functions mechanically as a docking factor within the peroxisomal matrix enabling the import of proteins into peroxisomes which are cellular organelles responsible for various metabolic functions.
- Biological function summary
PEX14 plays a significant role in maintaining peroxisome structure and function. It is part of the peroxisome importomer complex which facilitates the import of matrix proteins by interacting with peroxins such as PEX5. PEX14's interaction with these proteins ensures the proper translocation and sorting of enzymes critical for peroxisomal activities like fatty acid oxidation and reactive oxygen species detoxification.
- Pathways
PEX14 is involved in key metabolic and cellular pathways including peroxisomal fatty acid beta-oxidation and ether lipid metabolism. It directly associates with PEX5 a peroxin that transports cargo proteins. In these pathways PEX14 plays a central role in maintaining metabolic processes that are essential for lipid metabolism and cellular detoxification.
- Associated diseases and disorders
PEX14’s malfunction or absence can contribute to Peroxisome Biogenesis Disorders (PBDs) such as Zellweger syndrome. These disorders significantly affect cellular metabolism and result in severe developmental issues. PEX14's interactions with proteins like PEX19 affect peroxisomal membrane biogenesis further linking PEX14 to these metabolic disorders and highlighting its importance in peroxisomal function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-PEX14 antibody (ab183885)
10% SDS PAGE
All lanes: Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (ab183885)
Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells labeling PEX14 with ab183885 at 1/500 dilution (green) and Hoechst 33342 (blue). Cells were fixed in 4% paraformaldehyde at room temperature for 15 mins.
Western blot - Anti-PEX14 antibody (ab183885)
10% SDS-PAGE
All lanes: Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa
Western blot - Anti-PEX14 antibody (ab183885)
10% SDS-PAGE
All lanes: Western blot - Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa
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