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AB95959

Anti-PEX19 antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal PEX19 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PEX19 aa 1 to C-terminus.

View Alternative Names

HK33, PXF, OK/SW-cl.22, PEX19, Peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, Peroxin-19, Peroxisomal farnesylated protein

3 Images
Western blot - Anti-PEX19 antibody (AB95959)
  • WB

Supplier Data

Western blot - Anti-PEX19 antibody (AB95959)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-PEX19 antibody (ab95959) at 1/10000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 33 kDa

false

Western blot - Anti-PEX19 antibody (AB95959)
  • WB

Supplier Data

Western blot - Anti-PEX19 antibody (AB95959)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PEX19 antibody (ab95959) at 1/1000 dilution

Lane 1:

PC12 cell lysates at 30 µg

Lane 2:

Rat2 cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 33 kDa

false

Western blot - Anti-PEX19 antibody (AB95959)
  • WB

Supplier Data

Western blot - Anti-PEX19 antibody (AB95959)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-PEX19 antibody (ab95959) at 1/10000 dilution

All lanes:

Mouse liver tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PEX19 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P40855

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified by antigen affinity chromatography.
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX19 also known as peroxisomal biogenesis factor 19 is a protein important for the assembly of peroxisomal membranes. It has a molecular mass of approximately 36 kDa and is expressed across multiple tissues in the human body with particularly high levels in the liver and kidneys. This protein functions as a chaperone and is essential for the import of peroxisomal membrane proteins. It plays a significant role in the binding of these peroxisomal proteins and helps in their transportation to the peroxisomes.
Biological function summary

The protein plays a central role in peroxisome biogenesis facilitating the correct assembly and maintenance of the peroxisome. PEX19 interacts with other components in the peroxisomal import machinery such as PEX3 and PEX16 forming a complex responsible for the recognition and stabilization of peroxisomal membrane proteins. Through these interactions it ensures the functional integrity of peroxisomes which are important for metabolic tasks like fatty acid beta-oxidation and detoxification of hydrogen peroxide.

Pathways

PEX19 is involved in the peroxisomal protein import pathway and the broader lipid metabolism pathway. It plays an important role in peroxisomal membrane assembly through its interactions with PEX3 a receptor on the peroxisomal membrane that facilitates protein docking. This process is essential for maintaining efficient peroxisomal functions as disruptions may lead to metabolic imbalances involving lipids and reactive oxygen species.

PEX19 has significant implications in peroxisomal biogenesis disorders including Zellweger spectrum disorders. Mutations or deficiencies in PEX19 can lead to impaired peroxisome formation manifesting in severe metabolic dysfunctions. PEX19 is linked to disorders characterized by neurological deficits liver dysfunction and skeletal abnormalities. Its relationship with PEX3 is also critical in these disorders as any disruptions in their interaction can exacerbate the condition's severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
See full target information PEX19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational science 14:1490-1504 PubMed33742760

2021

Identification of small molecules that mitigate vincristine-induced neurotoxicity while sensitizing leukemia cells to vincristine.

Applications

Unspecified application

Species

Unspecified reactive species

Barthelemy Diouf,Claudia Wing,John C Panetta,Donnie Eddins,Wenwei Lin,Wenjian Yang,Yiping Fan,Deqing Pei,Cheng Cheng,Shannon M Delaney,Wei Zhang,Erik J Bonten,Kristine R Crews,Steven W Paugh,Lie Li,Burgess B Freeman,Robert J Autry,Jordan A Beard,Daniel C Ferguson,Laura J Janke,Kirsten K Ness,Taosheng Chen,Stanislav S Zakharenko,Sima Jeha,Ching-Hon Pui,Mary V Relling,M Eileen Dolan,William E Evans
View all publications
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Product promise

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