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AB137072

Anti-PEX19 antibody [EPR9266(B)]

4

(1 Review)

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(18 Publications)

Rabbit Recombinant Monoclonal PEX19 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 18 publications.

View Alternative Names

HK33, PXF, OK/SW-cl.22, PEX19, Peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, Peroxin-19, Peroxisomal farnesylated protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (AB137072)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1 : 100 (10.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (AB137072)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (AB137072)
  • IP

Lab

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

ab137072 (purified) at 1 : 50 dilution (2μg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
Lane 1 (input) : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10μg
Lane 2 (+) : ab137072 & MOLT-4 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Western blot - Anti-PEX19 antibody [EPR9266(B)] (AB137072)
  • WB

Lab

Western blot - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

The doublets are reported by PMID : 9339377, 28817674 and 10051604.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Rat brain lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 35 kDa,40 kDa

false

Western blot - Anti-PEX19 antibody [EPR9266(B)] (AB137072)
  • WB

Lab

Western blot - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

The doublets are reported by PMID : 9339377, 28817674 and 10051604.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 35 kDa,40 kDa

false

  • Carrier free

    Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9266(B)

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX19 also known as peroxisomal biogenesis factor 19 is a protein important for the assembly of peroxisomal membranes. It has a molecular mass of approximately 36 kDa and is expressed across multiple tissues in the human body with particularly high levels in the liver and kidneys. This protein functions as a chaperone and is essential for the import of peroxisomal membrane proteins. It plays a significant role in the binding of these peroxisomal proteins and helps in their transportation to the peroxisomes.
Biological function summary

The protein plays a central role in peroxisome biogenesis facilitating the correct assembly and maintenance of the peroxisome. PEX19 interacts with other components in the peroxisomal import machinery such as PEX3 and PEX16 forming a complex responsible for the recognition and stabilization of peroxisomal membrane proteins. Through these interactions it ensures the functional integrity of peroxisomes which are important for metabolic tasks like fatty acid beta-oxidation and detoxification of hydrogen peroxide.

Pathways

PEX19 is involved in the peroxisomal protein import pathway and the broader lipid metabolism pathway. It plays an important role in peroxisomal membrane assembly through its interactions with PEX3 a receptor on the peroxisomal membrane that facilitates protein docking. This process is essential for maintaining efficient peroxisomal functions as disruptions may lead to metabolic imbalances involving lipids and reactive oxygen species.

PEX19 has significant implications in peroxisomal biogenesis disorders including Zellweger spectrum disorders. Mutations or deficiencies in PEX19 can lead to impaired peroxisome formation manifesting in severe metabolic dysfunctions. PEX19 is linked to disorders characterized by neurological deficits liver dysfunction and skeletal abnormalities. Its relationship with PEX3 is also critical in these disorders as any disruptions in their interaction can exacerbate the condition's severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
See full target information PEX19
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu9104 PubMed40601736

2025

FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responses.

Applications

Unspecified application

Species

Unspecified reactive species

Choah Kim,Katlyn R Gabriel,Dylan Boone,Matthew R Brown,Katherine Oppenheimer,Maria Kost-Alimova,Juan Lorenzo B Pablo,Anna Greka

Npj viruses 2:6 PubMed40295745

2024

The Wnt/β-catenin pathway is important for replication of SARS-CoV-2 and other pathogenic RNA viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Zaikun Xu,Mohamed Elaish,Cheung Pang Wong,Bardes B Hassan,Joaquin Lopez-Orozco,Alberto Felix-Lopez,Natacha S Ogando,Les Nagata,Lara K Mahal,Anil Kumar,Joyce A Wilson,Ryan Noyce,Irv Mayers,Christopher Power,David Evans,Tom C Hobman

Autophagy 19:1781-1802 PubMed36541703

2022

PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Demers,Victoria Riccio,Doo Sin Jo,Sushil Bhandari,Kelsey B Law,Weifang Liao,Choy Kim,G Angus McQuibban,Seong-Kyu Choe,Dong-Hyung Cho,Peter K Kim

Frontiers in cell and developmental biology 10:859052 PubMed35557938

2022

PEX19 Coordinates Neutral Lipid Storage in Cells in a Peroxisome-Independent Fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Sven Lyschik,Anna A Lauer,Tanja Roth,Daniel Janitschke,Markus Hollander,Thorsten Will,Tobias Hartmann,Ron R Kopito,Volkhard Helms,Marcus O W Grimm,Bianca Schrul

Viruses 14: PubMed35215846

2022

Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif.

Applications

Unspecified application

Species

Unspecified reactive species

Mafalda A Farelo,Despoina Korrou-Karava,Katrina F Brooks,Tiffany A Russell,Kevin Maringer,Peter U Mayerhofer

Cells 10: PubMed34571897

2021

Hypothyroidism Intensifies Both Canonic and the Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Aleksic,Igor Golic,Andjelika Kalezic,Aleksandra Jankovic,Bato Korac,Aleksandra Korac

American journal of physiology. Endocrinology and metabolism 320:E1053-E1067 PubMed33843280

2021

Combined effects of a ketogenic diet and exercise training alter mitochondrial and peroxisomal substrate oxidative capacity in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Tai-Yu Huang,Melissa A Linden,Scott E Fuller,Felicia R Goldsmith,Jacob Simon,Heidi M Batdorf,Matthew C Scott,Nabil M Essajee,John M Brown,Robert C Noland

Science advances 6:eabb2210 PubMed32704543

2020

Increased lysosomal biomass is responsible for the resistance of triple-negative breast cancers to CDK4/6 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Fassl,Christopher Brain,Monther Abu-Remaileh,Iga Stukan,Deborah Butter,Piotr Stepien,Avery S Feit,Johann Bergholz,Wojciech Michowski,Tobias Otto,Qing Sheng,Alice Loo,Walter Michael,Ralph Tiedt,Carmine DeAngelis,Rachel Schiff,Baishan Jiang,Bojana Jovanovic,Karolina Nowak,Maria Ericsson,Michael Cameron,Nathanael Gray,Deborah Dillon,Jean J Zhao,David M Sabatini,Rinath Jeselsohn,Myles Brown,Kornelia Polyak,Piotr Sicinski

EMBO reports 21:e49865 PubMed31894645

2020

Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Covill-Cooke,Viktoriya S Toncheva,James Drew,Nicol Birsa,Guillermo López-Doménech,Josef T Kittler

Medicine and science in sports and exercise 52:37-48 PubMed31389908

2019

Response of Liver Metabolic Pathways to Ketogenic Diet and Exercise Are Not Additive.

Applications

Unspecified application

Species

Unspecified reactive species

Tai-Yu Huang,Felicia R Goldsmith,Scott E Fuller,Jacob Simon,Heidi M Batdorf,Matthew C Scott,Nabil M Essajee,John M Brown,David H Burk,Christopher D Morrison,Susan J Burke,J Jason Collier,Robert C Noland
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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