Rabbit Recombinant Monoclonal PEX19 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--immunocytochemistry-immunofluorescence-img86494.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)")
![Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--immunoprecipitation-img5899.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)")
![Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--flow-cytometry-intracellular-img414789.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (AB232421)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Expected | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
Alternative names
HK33, PXF, OK/SW-cl.22, PEX19, Peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, Peroxin-19, Peroxisomal farnesylated protein
Recommended products
Rabbit Recombinant Monoclonal PEX19 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9266(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232421 is the carrier-free version of Anti-PEX19 antibody [EPR9266(B)] ab137072.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEX19 also known as peroxisomal biogenesis factor 19 is a protein important for the assembly of peroxisomal membranes. It has a molecular mass of approximately 36 kDa and is expressed across multiple tissues in the human body with particularly high levels in the liver and kidneys. This protein functions as a chaperone and is essential for the import of peroxisomal membrane proteins. It plays a significant role in the binding of these peroxisomal proteins and helps in their transportation to the peroxisomes.
- Biological function summary
The protein plays a central role in peroxisome biogenesis facilitating the correct assembly and maintenance of the peroxisome. PEX19 interacts with other components in the peroxisomal import machinery such as PEX3 and PEX16 forming a complex responsible for the recognition and stabilization of peroxisomal membrane proteins. Through these interactions it ensures the functional integrity of peroxisomes which are important for metabolic tasks like fatty acid beta-oxidation and detoxification of hydrogen peroxide.
- Pathways
PEX19 is involved in the peroxisomal protein import pathway and the broader lipid metabolism pathway. It plays an important role in peroxisomal membrane assembly through its interactions with PEX3 a receptor on the peroxisomal membrane that facilitates protein docking. This process is essential for maintaining efficient peroxisomal functions as disruptions may lead to metabolic imbalances involving lipids and reactive oxygen species.
- Associated diseases and disorders
PEX19 has significant implications in peroxisomal biogenesis disorders including Zellweger spectrum disorders. Mutations or deficiencies in PEX19 can lead to impaired peroxisome formation manifesting in severe metabolic dysfunctions. PEX19 is linked to disorders characterized by neurological deficits liver dysfunction and skeletal abnormalities. Its relationship with PEX3 is also critical in these disorders as any disruptions in their interaction can exacerbate the condition's severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--immunocytochemistry-immunofluorescence-img86494.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)")
Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)
Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified Anti-PEX19 antibody [EPR9266(B)] ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PEX19 antibody [EPR9266(B)] ab137072).
![Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--immunoprecipitation-img5899.jpg "Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)")
Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)
Anti-PEX19 antibody [EPR9266(B)] ab137072 (purified) at 1:50 dilution (2μg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10μg
Lane 2 (+): Anti-PEX19 antibody [EPR9266(B)] ab137072 & MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PEX19 antibody [EPR9266(B)] ab137072 in MOLT-4 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PEX19 antibody [EPR9266(B)] ab137072).
All lanes: Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (Anti-PEX19 antibody [EPR9266(B)] ab137072)
Predicted band size: 33 kDa
Observed band size: 33 kDa
![Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421), expandable thumbnail](https://content.abcam.com/products/images/pex19-antibody-epr9266b-bsa-and-azide-free-ab232421--flow-cytometry-intracellular-img414789.jpg "Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)")
Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)
Intracellular flow cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling PEX19 with Anti-PEX19 antibody [EPR9266(B)] ab137072 (red) or a rabbit IgG (negative) (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PEX19 antibody [EPR9266(B)] ab137072).
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