Rabbit Polyclonal PEX3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PEX3 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PEX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56589
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.
Alternative names
Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3, PEX3
Recommended products
Rabbit Polyclonal PEX3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PEX3 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PEX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56589
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEX3 also known as Peroxisomal biogenesis factor 3 is a protein involved in peroxisome formation. PEX3 has a known amino acid sequence that gives it a molecular mass of approximately 45 kDa. It is mainly expressed in tissues with high metabolic activity such as liver kidney and heart. As a membrane protein PEX3 is located on the peroxisomal membrane and plays a direct role in the import of proteins into the peroxisome.
- Biological function summary
PEX3 is essential for the protein import machinery of peroxisomes. It acts as a receptor on the peroxisomal membrane interacting with other peroxisomal proteins including PEX19. This interaction helps in the assembly of peroxisomal membrane proteins. It is part of a larger protein complex necessary for the formation and maintenance of functional peroxisomes which are organelles responsible for various metabolic functions.
- Pathways
PEX3 plays a central role in peroxisome biogenesis. It functions within the peroxisomal protein import pathway an important process for the maintenance of peroxisomes. In this context PEX3 interacts closely with PEX19 which acts as a chaperone and receptor for newly synthesized peroxisomal membrane proteins. Together PEX3 and PEX19 facilitate the import and proper localization of these proteins into the peroxisomal membrane.
- Associated diseases and disorders
Mutations or deficiencies in PEX3 can lead to peroxisome biogenesis disorders (PBDs) such as Zellweger syndrome. This severe genetic disorder affects multiple systems and results from impaired peroxisomal formation and function. PEX1 another protein involved in peroxisome biogenesis also relates to these disorders emphasizing the importance of coordinated interactions between these proteins for proper cellular function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human skeletal muscle using ab247042 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human kidney tissue using ab247042 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human liver tissue using ab247042 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human testis tissue using ab247042 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human thyroid gland tissue using ab247042 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX3 antibody (ab247042)
Immunohistochemical analysis showing PEX3 staining in paraffin embedded Human adrenal gland tissue using ab247042 at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PEX3 antibody (ab247042)
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling PEX3 using ab247042 at 4 μg/ml (green) in ICC/IF.
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