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AB248640

Anti-PEX7 antibody [EPR7715(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PEX7 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PTS2R, PEX7, Peroxisomal targeting signal 2 receptor, PTS2 receptor, Peroxin-7

2 Images
Western blot - Anti-PEX7 antibody [EPR7715(2)(B)] - BSA and Azide free (AB248640)
  • WB

Unknown

Western blot - Anti-PEX7 antibody [EPR7715(2)(B)] - BSA and Azide free (AB248640)

This data was developed using ab133754, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PEX7 antibody [EPR7715(2)(B)] (<a href='/en-us/products/primary-antibodies/pex7-antibody-epr77152b-ab133754'>ab133754</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal muscle lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 36 kDa

false

OI-RD Scanning - Anti-PEX7 antibody [EPR7715(2)(B)] - BSA and Azide free (AB248640)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PEX7 antibody [EPR7715(2)(B)] - BSA and Azide free (AB248640)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7715(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248640 is the carrier-free version of ab133754.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEX7 also known as Peroxisomal Biogenesis Factor 7 functions as a receptor for peroxisomal targeting signal 2 (PTS2). PEX7 has a molecular mass of approximately 39 kDa and localizes primarily in the cytoplasm and peroxisomes of cells. It is abundantly expressed in a variety of tissues including the liver kidney and heart. The expression level suggests that PEX7 plays an important mechanical role in peroxisomal import processes.
Biological function summary

PEX7 facilitates the import of peroxisomal matrix proteins with PTS2 signals by forming a complex with them. This complex guides the proteins to the peroxisome important for maintaining peroxisomal function and integrity. PEX7 interacts with other peroxisomal biogenesis factors such as PEX5 and PEX14 during this process. Proper functioning of PEX7 ensures that enzymes needed for lipid metabolism and other peroxisomal activities are correctly imported to their destination.

Pathways

Peroxisomal protein import involves PEX7 which integrates into broader systems like the peroxisome biogenesis and lipid metabolism pathways. In these pathways PEX7 associates with PEX5 an important player in both pathways facilitating the import of important enzymes for beta-oxidation of fatty acids. Without the proper coordination of these proteins the pathways would falter leading to metabolic irregularities.

Malfunction of PEX7 correlates strongly with Rhizomelic Chondrodysplasia Punctata (RCDP) and Zellweger Spectrum Disorders (ZSD). In these conditions PEX7's interaction with proteins like PEX5 becomes compromised leading to impaired peroxisomal protein import and subsequent cellular dysfunction. The dysfunctions highlight the importance of PEX7 in maintaining normal cellular physiology and highlight its relevance in therapeutic research targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor required for the peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal (PubMed : 11931631, PubMed : 22057399, PubMed : 25538232, PubMed : 9090381). Specifically binds to cargo proteins containing a PTS2 peroxisomal targeting signal in the cytosol (PubMed : 11931631, PubMed : 22057399, PubMed : 25538232). Cargo protein-binding triggers interaction with PEX5 and formation of a ternary complex composed of PEX5 and PEX7 along with PTS2-containing cargo proteins, which is tranlocated into peroxisomes by passing through the PEX13-PEX14 docking complex (PubMed : 11546814, PubMed : 25538232).
See full target information PEX7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e727 PubMed36301031

2022

Forkhead box M1 recruits FoxP3 Treg cells to induce immune escape in hilar cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ma,Zhaowei Sun,Xueliang Li,Jingyun Guo,Qinlei Wang,Mujian Teng
View all publications
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Product promise

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