Rabbit Polyclonal PF4 antibody. Suitable for WB, sELISA and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PF4.
Images
Publications
Filter by
- International journal of molecular sciences 24:2022CXCL4-RNA Complexes Circulate in Systemic Sclerosis and Amplify Inflammatory/Pro-Fibrotic Responses by Myeloid Dendritic Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesImmacolata Pietraforte,Alessia Butera,Lucia Gaddini,Anna Mennella,Raffaella Palazzo,Doriana Campanile,Katia Stefanantoni,Valeria Riccieri,Roberto Lande,Loredana FrascaPubMed 36614095
- mAbs 13:18876282021Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLise I Loberg,Meha Chhaya,Alexander Ibraghimov,Edit Tarcsa,Andreas Striebinger,Andreas Popp,Lili Huang,Frank Oellien,Stefan BarghornPubMed 33596779
- Cell reports 28:896-908.e52019Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgostina Carestia,Hebe A Mena,Cinthia M Olexen,Juan M Ortiz Wilczyñski,Soledad Negrotto,Andrea E Errasti,Ricardo M Gómez,Craig N Jenne,Eugenio A Carrera Silva,Mirta SchattnerPubMed 31340152
- Nature communications 10:17312019CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-α production in systemic sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberto Lande et. al.PubMed 31043596
- Scientific reports 9:60592019Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPuangpaka Chanpeng et. al.PubMed 30988349
- JCI insight 3:2018Neutrophil accumulation and NET release contribute to thrombosis in HIT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKandace Gollomp et. al.PubMed 30232279
- The Journal of clinical endocrinology and metabolism 102:1851-18602017Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline A Maybin,Uma Thiruchelvam,Mayank Madhra,Philippa T K Saunders,Hilary O D CritchleyPubMed 28323919
- Arthritis research & therapy 18:1912016Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty.
- Molecular medicine reports 14:2473-822016Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiation.
- The Biochemical journal 422:533-422009Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domains.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PF4. Database link P02776
Consider this alternative
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Can be paired for Sandwich ELISA with ab83123 |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed:29930254, PubMed:9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed:18174362, PubMed:29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed:18174362, PubMed:24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed:9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed:29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed:35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed:34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed:9395524).
Alternative names
CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A
Recommended products
Rabbit Polyclonal PF4 antibody. Suitable for WB, sELISA and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PF4.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PF4. Database link P02776
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-PF4 antibody [RTO] ab49735
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
- Biological function summary
PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.
- Pathways
PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.
- Associated diseases and disorders
Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-PF4 antibody (ab9561)
All lanes: Western blot - Anti-PF4 antibody (ab9561) at 1 µg/mL
All lanes: Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa, 18 kDa, 30 kDa, 37 kDa
Exposure time: 2min
Sandwich ELISA - Anti-PF4 antibody (ab9561)
To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com