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AB9561

Anti-PF4 antibody

4

(1 Review)

|

(14 Publications)

Rabbit Polyclonal PF4 antibody. Suitable for WB, sELISA and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PF4.

View Alternative Names

CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

2 Images
Sandwich ELISA - Anti-PF4 antibody (AB9561)
  • sELISA

Unknown

Sandwich ELISA - Anti-PF4 antibody (AB9561)

To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.

Western blot - Anti-PF4 antibody (AB9561)
  • WB

Unknown

Western blot - Anti-PF4 antibody (AB9561)

All lanes:

Western blot - Anti-PF4 antibody (ab9561) at 1 µg/mL

All lanes:

Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa,18 kDa,30 kDa,37 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PF4.

P02776

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Alternative Products

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Complementary Products

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Assay Kits

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Primary Antibodies

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Primary Antibodies

AB133347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Can be paired for Sandwich ELISA with <a href='/en-us/products/unavailable/pf4-antibody-biotin-ab83123'>ab83123</a></p>" } } }
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Product details

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab49735
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
See full target information PF4
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 25:1322-1334 PubMed39601768

2024

PF4 regulates neuronal ferroptosis in cerebral hemorrhage through CXCR3/PI3K/AKT/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Hu,Yunfeng Li,Guohong Zhang,Wei Wang,Liping An,Ran An,Yu Liu

International journal of molecular sciences 24: PubMed36614095

2022

CXCL4-RNA Complexes Circulate in Systemic Sclerosis and Amplify Inflammatory/Pro-Fibrotic Responses by Myeloid Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Immacolata Pietraforte,Alessia Butera,Lucia Gaddini,Anna Mennella,Raffaella Palazzo,Doriana Campanile,Katia Stefanantoni,Valeria Riccieri,Roberto Lande,Loredana Frasca

mAbs 13:1887628 PubMed33596779

2021

Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Lise I Loberg,Meha Chhaya,Alexander Ibraghimov,Edit Tarcsa,Andreas Striebinger,Andreas Popp,Lili Huang,Frank Oellien,Stefan Barghorn

Annals of palliative medicine 9:2723-2730 PubMed32954739

2020

The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Shuyu Ge

Cell reports 28:896-908.e5 PubMed31340152

2019

Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Agostina Carestia,Hebe A Mena,Cinthia M Olexen,Juan M Ortiz Wilczyñski,Soledad Negrotto,Andrea E Errasti,Ricardo M Gómez,Craig N Jenne,Eugenio A Carrera Silva,Mirta Schattner

Nature communications 10:1731 PubMed31043596

2019

CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-α production in systemic sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Lande,Ernest Y Lee,Raffaella Palazzo,Barbara Marinari,Immacolata Pietraforte,Giancarlo Santiago Santos,Yves Mattenberger,Francesca Spadaro,Katia Stefanantoni,Nicoletta Iannace,Aleksandra Maria Dufour,Mario Falchi,Manuela Bianco,Elisabetta Botti,Luca Bianchi,Montserrat Alvarez,Valeria Riccieri,Marie-Elise Truchetet,Gerard C L Wong,Carlo Chizzolini,Loredana Frasca

Scientific reports 9:6059 PubMed30988349

2019

Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patients.

Applications

Unspecified application

Species

Unspecified reactive species

Puangpaka Chanpeng,Saovaros Svasti,Kittiphong Paiboonsukwong,Duncan R Smith,Kamonlak Leecharoenkiat

JCI insight 3: PubMed30232279

2018

Neutrophil accumulation and NET release contribute to thrombosis in HIT.

Applications

Unspecified application

Species

Unspecified reactive species

Kandace Gollomp,Minna Kim,Ian Johnston,Vincent Hayes,John Welsh,Gowthami M Arepally,Mark Kahn,Michele P Lambert,Adam Cuker,Douglas B Cines,Lubica Rauova,M Anna Kowalska,Mortimer Poncz

The Journal of clinical endocrinology and metabolism 102:1851-1860 PubMed28323919

2017

Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline A Maybin,Uma Thiruchelvam,Mayank Madhra,Philippa T K Saunders,Hilary O D Critchley

Arthritis research & therapy 18:191 PubMed27558507

2016

Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty.

Applications

Unspecified application

Species

Human

Masahiro Izumi,Tatsuya Sakai,Atsunori Shirakawa,Hideko Kozuru,Yuka Jiuchi,Yasumori Izumi,Tomohiko Asahara,Kenji Kumagai,Masaaki Mawatari,Makoto Osaki,Satoru Motokawa,Kiyoshi Migita
View all publications
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Product promise

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