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Rabbit Recombinant Monoclonal PF4 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (AB303494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (AB303494), expandable thumbnail
  • Western blot - Anti-PF4 antibody [EPR26306-65] (AB303494), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PF4 antibody [EPR26306-65] (AB303494), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PF4 antibody [EPR26306-65] (AB303494), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Target data

Function

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity.

Alternative names

Cxcl4, Scyb4, Pf4, Platelet factor 4, PF-4, C-X-C motif chemokine 4

Recommended products

Rabbit Recombinant Monoclonal PF4 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26306-65
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.

Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Associated diseases and disorders

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from PF4 knockout mice labeling PF4 with ab303494 at 1/5000 dilution (0.11 µg/mL), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Antigen retrieval  was heat mediated  with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Counterstain was hematoxylin.

    Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from PF4 knockout mice.
    The section was incubated with ab303494 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID: T011518).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Immunohistochemical analysis of paraffin-embedded ((A) Bone marrow tissue from wild-type C57BL/6JGpt mice (B) Bone marrow tissue from PF4 knockout mice labeling PF4 with ab303494 at 1/5000 dilution (0.11 µg/mL), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Antigen retrieval  was heat mediated  with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Counterstain was hematoxylin.

    Positive staining on (A) Bone marrow tissue from wild-type C57BL/6JGpt mice and no staining on (B) Bone marrow tissue from PF4 knockout mice.
    The section was incubated with ab303494 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID: T011518).

  • Western blot - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Western blot - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1:200000) (36KDa)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID: T011518).

    Lanes 1 - 8: Western blot - Anti-PF4 antibody [EPR26306-65] (ab303494) at 1/1000 dilution

    Lanes 1 - 8: Western blot - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (Anti-PF4 antibody [EPR26306-65] - BSA and Azide free ab303495)

    Lane 1: Wild-type mouse spleen tissue lysate (male)

    Lane 2: PF4 knockout spleen tissue lysate (male)

    Lane 3: Wild-type mouse spleen tissue lysate (female)

    Lane 4: PF4 knockout spleen tissue lysate (female)

    Lane 5: Wild-type mouse bone marrow tissue lysate (male)

    Lane 6: PF4 knockout bone marrow tissue lysate (male)

    Lane 7: Wild-type mouse bone marrow tissue lysate (female)

    Lane 8: PF4 knockout bone marrow tissue lysate (female)

    Exposure time: 38s

  • Flow Cytometry (Intracellular) - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse blood cells cells labelling PF4 with ab303494 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD41 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab303494.

  • Immunocytochemistry/ Immunofluorescence - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling PF4 with ab303494 at 1/50 (9.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in subsets of mouse splenocytes. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling PF4 with ab303494 at 1/5000 (0.09 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on mouse pancreas.The section was incubated with ab303494 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] (ab303494)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PF4 with ab303494 at 1/5000 (0.09 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on megakaryocytes and platelets of mouse spleen (PMID: 11468158). The section was incubated with ab303494 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

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