Anti-PF4 antibody [EPR26306-65] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PF4 with ab303494 at 1/5000 (0.09 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on megakaryocytes and platelets of mouse spleen (PMID : 11468158). The section was incubated with ab303494 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling PF4 with ab303494 at 1/5000 (0.09 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : No staining on mouse pancreas.The section was incubated with ab303494 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling PF4 with ab303494 at 1/50 (9.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in subsets of mouse splenocytes. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse blood cells cells labelling PF4 with ab303494 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD41 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab303494.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded ((A) Bone marrow tissue from wild-type C57BL/6JGpt mice (B) Bone marrow tissue from PF4 knockout mice labeling PF4 with ab303494 at 1/5000 dilution (0.11 µg/mL), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Antigen retrieval  was heat mediated  with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Counterstain was hematoxylin. Positive staining on (A) Bone marrow tissue from wild-type C57BL/6JGpt mice and no staining on (B) Bone marrow tissue from PF4 knockout mice. The section was incubated with ab303494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID : T011518).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from PF4 knockout mice labeling PF4 with ab303494 at 1/5000 dilution (0.11 µg/mL), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Antigen retrieval  was heat mediated  with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Counterstain was hematoxylin. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from PF4 knockout mice. The section was incubated with ab303494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID : T011518).

• WB

Lab

Western blot - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (AB303495)

This data was developed using ab303494, the same antibody clone in a different buffer formulation. Loading control : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa) Blocking/Diluting buffer and concentration : 5% NFDM/TBST The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Pf4-KO homozygous mice (Strain ID : T011518).

Lanes 1 - 8:

Western blot - Anti-PF4 antibody [EPR26306-65] (<a href='/en-us/products/primary-antibodies/pf4-antibody-epr26306-65-ab303494'>ab303494</a>) at 1/1000 dilution

Lanes 1 - 8:

Western blot - Anti-PF4 antibody [EPR26306-65] - BSA and Azide free (ab303495)

Lane 1:

Wild-type mouse spleen tissue lysate (male)

Lane 2:

PF4 knockout spleen tissue lysate (male)

Lane 3:

Wild-type mouse spleen tissue lysate (female)

Lane 4:

PF4 knockout spleen tissue lysate (female)

Lane 5:

Wild-type mouse bone marrow tissue lysate (male)

Lane 6:

PF4 knockout bone marrow tissue lysate (male)

Lane 7:

Wild-type mouse bone marrow tissue lysate (female)

Lane 8:

PF4 knockout bone marrow tissue lysate (female)

false

Exposure time: 38s