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AB49735

Anti-PF4 antibody [RTO]

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(8 Publications)

Mouse Monoclonal PF4 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

1 Images
Western blot - Anti-PF4 antibody [RTO] (AB49735)
  • WB

Project

Western blot - Anti-PF4 antibody [RTO] (AB49735)

All lanes:

Western blot - Anti-PF4 antibody [RTO] (ab49735) at 1 µg/mL

All lanes:

Human Platelet (Human adult normal cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RTO

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
See full target information PF4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

mAbs 13:1887628 PubMed33596779

2021

Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Lise I Loberg,Meha Chhaya,Alexander Ibraghimov,Edit Tarcsa,Andreas Striebinger,Andreas Popp,Lili Huang,Frank Oellien,Stefan Barghorn

Annals of palliative medicine 9:2723-2730 PubMed32954739

2020

The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Shuyu Ge

Journal of cellular physiology 234:22378-22385 PubMed31073998

2019

Effects of CXCL4/CXCR3 on the lipopolysaccharide-induced injury in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Wang,Zhenzhen Zhao,Kaimin Zhu,Rui Bao,Yan Meng,Jinjun Bian,Xiaojian Wan,Tao Yang

PloS one 7:e43793 PubMed22916307

2012

Angiostatic factors in the pulmonary endarterectomy material from chronic thromboembolic pulmonary hypertension patients cause endothelial dysfunction.

Applications

IHC

Species

Unspecified reactive species

Diana Zabini,Chandran Nagaraj,Elvira Stacher,Irene M Lang,Patrick Nierlich,Walter Klepetko,Akos Heinemann,Horst Olschewski,Zoltán Bálint,Andrea Olschewski

Cancer 117:207-17 PubMed20806347

2010

Elevated expression of CXC chemokines in pediatric osteosarcoma patients.

Applications

IHC

Species

Human

Yiting Li,Ricardo Flores,Alexander Yu,M Fatih Okcu,Jeffrey Murray,Murali Chintagumpala,John Hicks,Ching C Lau,Tsz-Kwong Man

Proteome science 7:7 PubMed19291297

2009

Discovery and identification of potential biomarkers of pediatric acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Linan Shi,Jun Zhang,Peng Wu,Kai Feng,Jing Li,Zhensheng Xie,Peng Xue,Tanxi Cai,Ziyou Cui,Xiulan Chen,Junjie Hou,Jianzhong Zhang,Fuquan Yang

Blood 98:2442-7 PubMed11588041

2001

Heparin-induced thrombocytopenia/thrombosis in a transgenic mouse model requires human platelet factor 4 and platelet activation through FcgammaRIIA.

Applications

Unspecified application

Species

Unspecified reactive species

M P Reilly,S M Taylor,N K Hartman,G M Arepally,B S Sachais,D B Cines,M Poncz,S E McKenzie

Blood 95:1533-40 PubMed10688805

2000

Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

G M Arepally,S Kamei,K S Park,K Kamei,Z Q Li,W Liu,D L Siegel,W Kisiel,D B Cines,M Poncz
View all publications
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