AB184984

Anti-PFD6 antibody

Name: Anti-PFD6 antibody (ab184984) | Abcam
Brand: Abcam Limited
SKU: AB184984

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(2 Publications)

Rabbit Polyclonal PFD6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFDN6 aa 50 to C-terminus.

View Alternative Names

HKE2, PFD6, PFDN6, Prefoldin subunit 6, Protein Ke2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human liver tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling PFG6 at the nucleus and the Golgi apparatus with ab184984 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human testis tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human pancreas tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human colon tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PFD6 antibody (AB184984)

All lanes:

Western blot - Anti-PFD6 antibody (ab184984) at 1/500 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) whole cell lysate

Predicted band size: 14 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PFDN6 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15212

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFD6 also known as Prefoldin Subunit 6 is a component of the prefoldin complex. It has a molecular mass of approximately 15 kDa. PFD6 is expressed in various tissues including the heart liver and skeletal muscle. Mechanically PFD6 works as a molecular chaperone binding to newly synthesized polypeptides. It helps stabilize these nascent chains preventing premature folding and facilitating their transport to other complexes like the cytosolic chaperonin-containing TCP-1.

Biological function summary

PFD6 as part of the prefoldin complex assists in the correct folding of proteins necessary for cell function. The prefoldin complex acts as an assembly platform. It specifically interacts with cytoskeletal proteins such as actin and tubulin. This interaction is significant for maintaining cellular structure and dynamics. PFD6 therefore supports numerous cellular processes dependent on microtubule and actin filament formation.

Pathways

PFD6 participates in cellular maintenance processes. It plays a role in the protein folding and stabilization pathways working together with the cytosolic chaperonin CCT/TRiC complex. This interaction ensures that proteins involved in cell division and signaling like tubulins and actins are correctly folded. Additionally PFD6 connects to pathways involving cell motility and organization through stable support to the cytoskeleton.

PFD6 has links to certain neurodegenerative diseases and cancer. Aberrations in protein folding mechanisms can lead to accumulation of misfolded proteins contributing to neurodegenerative diseases like Alzheimer's. In cancer abnormal expression of PFD6 might affect cell structure and division due to its interactions with cytoskeletal elements like actin and tubulin. Research continues to explore how PFD6-related pathways influence these diseases highlighting potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

See full target information PFDN6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 47:52-62 PubMed36300673

2022

Prefoldin 6 promotes glioma progression via the AKT signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianglei Jiang,Jun Liu

Clinical epigenetics 8:48 PubMed27152124

2016

DNA methylation profiling identifies novel markers of progression in hepatitis B-related chronic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Müjdat Zeybel,Sezgin Vatansever,Timothy Hardy,Ayşegül Akder Sarı,Fulya Cakalağaoğlu,Arzu Avcı,Gemma Louise Zeybel,Serçin Karahüseyinoğlu,Matthew Bashton,John C Mathers,Belkıs Ünsal,Jelena Mann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFD6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Western blot - Anti-PFD6 antibody (AB184984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise