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Rabbit Recombinant Monoclonal PFDN1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (AB151708), expandable thumbnail
  • Western blot - Anti-PFDN1 antibody [EPR8547] (AB151708), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (AB151708), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] (AB151708), expandable thumbnail
  • Western blot - Anti-PFDN1 antibody [EPR8547] (AB151708), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Expected
Mouse
Expected
Tested
Not recommended
Expected
Rat
Expected
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100 - 1/500
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Alternative names

PFD1, PFDN1, Prefoldin subunit 1

Recommended products

Rabbit Recombinant Monoclonal PFDN1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8547
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The PFDN1 protein also known as prefoldin subunit 1 functions as part of the chaperone pathway in protein folding. It helps in the stabilization of nascent polypeptides facilitating their correct folding into functional structures. The molecular mass of PFDN1 is approximately 15 kDa. Expression of PFDN1 occurs in various tissues which include both highly proliferative and differentiated cells. The protein has a wider tissue distribution suggesting its fundamental role in several cellular processes.

Biological function summary

The prefoldin complex which includes PFDN1 assists in the proper folding of actin and tubulin proteins. This complex functions as a co-chaperone directing unfolded proteins to the cytosolic chaperonin TriC/CCT complex for further refolding. By interacting with actin and tubulin PFDN1 ensures that key structural proteins maintain their integrity and availability for cellular functions. This interaction plays a critical role in cytoskeleton dynamics affecting cellular shape and movement.

Pathways

PFDN1 plays a significant role in the protein folding pathway and the regulation of actin cytoskeleton pathway. Within these pathways PFDN1 helps coordinate with other proteins such as TCP1 part of the TriC/CCT complex. This cooperation ensures efficient protein maturation and structural arrangement within cells. The correct functioning of these pathways relies on well-folded actin and tubulin proteins impacting cellular architecture and intracellular transport mechanisms.

Associated diseases and disorders

Malfunction of PFDN1 can contribute to neurodegenerative diseases and cancer. The disruption of proper protein folding might result in aggregates characteristic of neurodegenerative conditions. Proteins like APOE have been studied in relation to how PFDN1 disruptions might contribute to neurodegenerative disease pathways. In cancer altered PFDN1 expression affects cell proliferation and survival. This alteration connects with oncogenes and tumor suppressor genes influencing tumor growth and progression. Understanding these associations provides insight into the therapeutic targeting of PFDN1 in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PFDN1 with ab151708 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708), expandable thumbnail

    Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708)

    All lanes: Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708) at 1/1000 dilution

    Lane 1: Human fetal brain tissue lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: HT1080 cell lysate at 10 µg

    Lane 4: PC12 cell lysate at 10 µg

    Lane 5: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PFDN1 with ab151708 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] (ab151708), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] (ab151708)

    Intracellular flow cytometric analysis of permeabilized PC12 cells labeling PFDN1 with ab151708 at 1/100 dilution (red). Rabbit IgG negative control (green).

  • Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708)

    PFDN1 western blot using anti-PFDN1 antibody [EPR8547] ab151708. Publication image and figure legend from Gao, Q., Lei, F., et al., 2020, Int J Biol Sci, PubMed 32140072.


    ab151708 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab151708 please see the product overview.

    Direct targeting of miR-2277-3p and miR-26b-3p on NUPR1L and PFDN1. (a) Luciferase reporter vectors containing wild-type or mutated 3'-UTR of NUPR1L were constructed to test direct targeting of miR-2277-3p on NUPR1L mRNA. (b) Luciferase reporter vectors containing wild-type or mutated 3'-UTR of PFDN1 were const. Ructed to test direct targeting of miR-26b-3p on PFDN1 mRNA. (c) The effect of miR-2277-3p on mRNA and protein expression of NUPR1L was examined by qPCR and Western blot. (d) The effect of miR-26b-3p on mRNA and protein expression of PFDN1 was examined by qPCR and Western blot. Data are the means ± SD. of three independent experiments. *P < 0.05 and ** P<0.01 versus control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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