AB249028

Anti-PFDN1 antibody [EPR8547] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PFDN1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

PFD1, PFDN1, Prefoldin subunit 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

This data was developed using ab151708, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PFDN1 with ab151708 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

This data was developed using ab151708, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PFDN1 with ab151708 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

This data was developed using ab151708, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized PC12 cells labeling PFDN1 with ab151708 at 1/100 dilution (red). Rabbit IgG negative control (green).

Unknown

Western blot - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

This data was developed using ab151708, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PFDN1 antibody [EPR8547] (<a href='/en-us/products/primary-antibodies/pfdn1-antibody-epr8547-ab151708'>ab151708</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HT1080 cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Lane 5:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 14 kDa

false

Unconjugated

Anti-PFDN1 antibody [EPR8547]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8547

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249028 is the carrier-free version of ab151708.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PFDN1 protein also known as prefoldin subunit 1 functions as part of the chaperone pathway in protein folding. It helps in the stabilization of nascent polypeptides facilitating their correct folding into functional structures. The molecular mass of PFDN1 is approximately 15 kDa. Expression of PFDN1 occurs in various tissues which include both highly proliferative and differentiated cells. The protein has a wider tissue distribution suggesting its fundamental role in several cellular processes.

Biological function summary

The prefoldin complex which includes PFDN1 assists in the proper folding of actin and tubulin proteins. This complex functions as a co-chaperone directing unfolded proteins to the cytosolic chaperonin TriC/CCT complex for further refolding. By interacting with actin and tubulin PFDN1 ensures that key structural proteins maintain their integrity and availability for cellular functions. This interaction plays a critical role in cytoskeleton dynamics affecting cellular shape and movement.

Pathways

PFDN1 plays a significant role in the protein folding pathway and the regulation of actin cytoskeleton pathway. Within these pathways PFDN1 helps coordinate with other proteins such as TCP1 part of the TriC/CCT complex. This cooperation ensures efficient protein maturation and structural arrangement within cells. The correct functioning of these pathways relies on well-folded actin and tubulin proteins impacting cellular architecture and intracellular transport mechanisms.

Malfunction of PFDN1 can contribute to neurodegenerative diseases and cancer. The disruption of proper protein folding might result in aggregates characteristic of neurodegenerative conditions. Proteins like APOE have been studied in relation to how PFDN1 disruptions might contribute to neurodegenerative disease pathways. In cancer altered PFDN1 expression affects cell proliferation and survival. This alteration connects with oncogenes and tumor suppressor genes influencing tumor growth and progression. Understanding these associations provides insight into the therapeutic targeting of PFDN1 in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

See full target information PFDN1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFDN1 antibody [EPR8547] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

Western blot - Anti-PFDN1 antibody [EPR8547] - BSA and Azide free (AB249028)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Proteins & Peptides

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Product promise