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AB237534

Anti-PFDN2 antibody

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(2 Publications)

Rabbit Polyclonal PFDN2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PFDN2.

View Alternative Names

PFD2, HSPC231, PFDN2, Prefoldin subunit 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PFDN2 antibody (AB237534)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFDN2 antibody (AB237534)

PC-3 (human prostate adenocarcinoma cell line) cells stained for PFDN2 (green) using ab237534 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN2 antibody (AB237534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN2 antibody (AB237534)

Paraffin-embedded human pancreas tissue stained for PFDN2 with ab237534 at a 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PFDN2 antibody (AB237534)
  • WB

Supplier Data

Western blot - Anti-PFDN2 antibody (AB237534)

All lanes:

Western blot - Anti-PFDN2 antibody (ab237534) at 1/500 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PFDN2.

Q9UHV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The prefoldin subunit 2 protein also known as PFDN2 plays an important role in chaperone-mediated protein folding. The protein has an approximate mass of 16 kDa. Scientists have observed its expression in many tissues including the brain liver and muscle. PFDN2 forms part of the prefoldin complex which participates in assisting nascent protein chains to fold correctly.
Biological function summary

PFDN2 functions to stabilize unfolding proteins by housing them in its prefoldin complex until they achieve proper conformation. This complex interacts directly with the cytoskeleton to maintain cellular architecture. The prefoldin complex consists of six subunits including PFDN2 that work together to perform this chaperone role effectively.

Pathways

PFDN2 contributes significantly to both the tubulin and actin folding pathways. This involves its interaction with chaperonins including CCT (chaperonin-containing TCP-1) important for the assembly of the cytoskeleton. It's vital in managing protein dynamics thereby influencing cellular activities like motility and division through its collaborative action with these pathways.

PFDN2 has associations with neurodegenerative conditions such as spinal muscular atrophy and amyotrophic lateral sclerosis. Researchers often study its interactions with other proteins such as SMN1 and SOD1 involved in these diseases. Dysregulation or mutations within PFDN2-related pathways could contribute to the progression of these disorders making it an area of active investigation for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
See full target information PFDN2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:801687 PubMed35096829

2022

Identification and Validation of Key Genes of Differential Correlations in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tingna Chen,Qiuming He,Zhenxian Xiang,Rongzhang Dou,Bin Xiong

OncoTargets and therapy 14:1007-1020 PubMed33603407

2021

Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Qiang Li,Ge Huang,Bing-Yao Lin,Dongzi Lin,Yan Ma,Zhao Zhang,Tao Chen,Jie Zhou
View all publications
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