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AB154573

Anti-PFKFB1 antibody

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(1 Publication)

Rabbit Polyclonal PFKFB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB1 aa 1-200.

View Alternative Names

F6PK, PFRX, PFKFB1, PFK/FBPase 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (AB154573)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (AB154573)

Immunohistochemical analysis of paraffin-embedded H358 xenograft labeling PFKFB1 with ab154573 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (AB154573)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (AB154573)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling PFKFB1 with ab154573 at 1/200 dilution (Green). Lower panel co-stained with Hoechst 33342 (blue).

Western blot - Anti-PFKFB1 antibody (AB154573)
  • WB

Unknown

Western blot - Anti-PFKFB1 antibody (AB154573)

10% SDS PAGE

All lanes:

Western blot - Anti-PFKFB1 antibody (ab154573) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

Hela whole cell lysate at 30 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PFKFB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P16118

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer (pH8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKFB1 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 1 plays an important role in glycolysis and gluconeogenesis. It catalyzes the synthesis and degradation of fructose-26-bisphosphate (F26BP) a potent regulator of the glycolytic flux. This protein has an approximate mass of 58-60 kDa and shows expression predominantly in liver and adipose tissue. Its distinct structure allows it to switch between kinase and phosphatase activities.
Biological function summary

PFKFB1 modulates the balance of carbohydrate metabolism. The protein manages levels of F26BP facilitating enhanced glycolysis or gluconeogenesis depending on metabolic demands. PFKFB1 does not form part of a larger enzymatic complex but it operates by itself to regulate metabolic processes. Its regulation involves allosteric changes and phosphorylation by other enzymes reflecting its importance in maintaining energy homeostasis.

Pathways

PFKFB1 features in both glycolytic and gluconeogenic pathways. The glycolysis pathway sees PFKFB1 influencing how cells convert glucose into pyruvate generating ATP. In the gluconeogenesis pathway PFKFB1 also contributes to converting non-carbohydrates into glucose. It interacts with proteins such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase-1) key regulators of the metabolic flux alongside PFKFB1.

Metabolic dysregulation involving PFKFB1 could relate to conditions like diabetes and metabolic syndrome. Abnormal PFKFB1 activity might affect glycolytic and gluconeogenic balance impacting glucose levels and energy management. It connects to insulin influencing insulin resistance and subsequent glucose uptake. Other proteins such as AKT and AMP-activated protein kinase (AMPK) interact with PFKFB1 during these conditions affecting its regulatory capacity on metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Synthesis and degradation of fructose 2,6-bisphosphate.
See full target information PFKFB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 65:101584 PubMed36055580

2022

PACAP attenuates hepatic lipid accumulation through the FAIM/AMPK/IRβ axis during overnutrition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Luo,Jiaxin Dai,Jianmin Liu,Yongmei Huang,Ziqiong Zheng,Pei Xu,Yi Ma
View all publications
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Product promise

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