AB155564

Anti-PFKFB1 antibody

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(4 Publications)

Rabbit Polyclonal PFKFB1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

PFK/FBPase 1, Pfkfb1

3 Images

Lab

Western blot - Anti-PFKFB1 antibody (AB155564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155564 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

All lanes:

Western blot - Anti-PFKFB1 antibody (ab155564) at 1 µg/mL

Lane 1:

Liver (Mouse) Tissue Lysate at 25 µg

Lane 2:

Liver (Rat) Tissue Lysate at 25 µg

Lane 3:

Liver (Human) Tissue Lysate - adult normal tissue at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

true

Exposure time: 16s

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (AB155564)

ICC/IF

Ap18717****

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (AB155564)

ICC/IF image of ab155564 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155564 at 5μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (AB155564)

IHC-P

Ap18717****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (AB155564)

IHC image of TPFKFB1 staining in Mouse normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab155564, 1μg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKFB1 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 1 plays an important role in glycolysis and gluconeogenesis. It catalyzes the synthesis and degradation of fructose-26-bisphosphate (F26BP) a potent regulator of the glycolytic flux. This protein has an approximate mass of 58-60 kDa and shows expression predominantly in liver and adipose tissue. Its distinct structure allows it to switch between kinase and phosphatase activities.

Biological function summary

PFKFB1 modulates the balance of carbohydrate metabolism. The protein manages levels of F26BP facilitating enhanced glycolysis or gluconeogenesis depending on metabolic demands. PFKFB1 does not form part of a larger enzymatic complex but it operates by itself to regulate metabolic processes. Its regulation involves allosteric changes and phosphorylation by other enzymes reflecting its importance in maintaining energy homeostasis.

Pathways

PFKFB1 features in both glycolytic and gluconeogenic pathways. The glycolysis pathway sees PFKFB1 influencing how cells convert glucose into pyruvate generating ATP. In the gluconeogenesis pathway PFKFB1 also contributes to converting non-carbohydrates into glucose. It interacts with proteins such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase-1) key regulators of the metabolic flux alongside PFKFB1.

Metabolic dysregulation involving PFKFB1 could relate to conditions like diabetes and metabolic syndrome. Abnormal PFKFB1 activity might affect glycolytic and gluconeogenic balance impacting glucose levels and energy management. It connects to insulin influencing insulin resistance and subsequent glucose uptake. Other proteins such as AKT and AMP-activated protein kinase (AMPK) interact with PFKFB1 during these conditions affecting its regulatory capacity on metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Synthesis and degradation of fructose 2,6-bisphosphate.

See full target information Pfkfb1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:311 PubMed40801925

2025

Quercetin alleviates acute pancreatitis by modulating glycolysis and mitochondrial function via PFKFB3 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Jiang,Jia Liu,Zhipeng Xu,Qi Song,Junjie Tao,Heng Zhu,Qiliang Li,Lei Li

Nature 646:198-207 PubMed40739344

2025

Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entry.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Paul,Derek L Bolhuis,Hualong Yan,Sudipto Das,Xia Xu,Christina C Abbate,Lisa M M Jenkins,Michael J Emanuele,Thorkell Andresson,Jing Huang,John G Albeck,Nicholas G Brown,Steven D Cappell

Cancer cell international 21:620 PubMed34819098

2021

IMUP and GPRC5A: two newly identified risk score indicators in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Wei,Guoye Qi,Zixin Zeng,Ningning Shen,Ziyue Wang,Honghong Shen,Lifang Gao,Chen Song,Wenxia Ma,Chen Wang

Antioxidants (Basel, Switzerland) 10: PubMed34679684

2021

Hsp22 Deficiency Induces Age-Dependent Cardiac Dilation and Dysfunction by Impairing Autophagy, Metabolism, and Oxidative Response.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Wu,Xiaonan Sun,Xiaomeng Shi,Lo Lai,Charles Wang,Mingxin Xie,Gangjian Qin,Hongyu Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKFB1 antibody

Western blot - Anti-PFKFB1 antibody (AB155564)

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (AB155564)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (AB155564)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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