AB234865

Anti-PFKFB2 antibody

Name: Anti-PFKFB2 antibody (ab234865) | Abcam
Brand: Abcam Limited
SKU: AB234865
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal PFKFB2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB2 aa 350 to C-terminus.

View Alternative Names

PFK/FBPase 2, PFKFB2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB2 antibody (AB234865)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB2 antibody (AB234865)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PFKFB2 (green) using ab234865 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB2 antibody (AB234865)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB2 antibody (AB234865)

Paraffin-embedded human lung cancer tissue stained for PFKFB2 using ab234865 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PFKFB2 antibody (AB234865)

All lanes:

Western blot - Anti-PFKFB2 antibody (ab234865) at 1/200 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 2:

Rat kidney lysate

Lane 3:

Rat brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PFKFB2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60825

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphofructokinase/fructose-26-bisphosphatase 2 often called PFKFB2 plays a significant role in the regulation of glycolysis and gluconeogenesis. This bifunctional enzyme has a molecular mass of approximately 55 kDa. PFKFB2 catalyzes the conversion between fructose-6-phosphate and fructose-26-bisphosphate. It is mainly expressed in cardiac tissue though expression has been noticed in other tissues such as skeletal muscle and liver.

Biological function summary

PFKFB2 regulates the levels of fructose-26-bisphosphate an important metabolic signal molecule. This molecule modulates the activity of key enzymes in glycolysis. PFKFB2 forms part of a complex that controls the glycolytic flux in response to hormonal and nutritional changes. By increasing levels of fructose-26-bisphosphate it strongly activates phosphofructokinase-1 (PFK-1) pushing cells towards glycolytic metabolism under conditions of increased energy demand.

Pathways

PFKFB2 directly influences the glycolysis and gluconeogenesis pathways. The regulation of these pathways by PFKFB2 ties it closely to energy metabolism. Additionally it has functional connections with enzymes such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase) helping to balance the glycolytic and gluconeogenic flux according to cellular needs.

Studies implicate PFKFB2 in conditions such as ischemic heart disease and certain cancers. Ischemic heart disease involves energy metabolism disruption where PFKFB2 plays a role in sustaining cardiac energy supply under oxygen-limited conditions. In cancer PFKFB2's altered expression correlates with the Warburg effect where cancer cells favor glycolysis even in the presence of oxygen. This connection makes PFKFB2 relevant to research fields exploring metabolic interventions in cancer therapeutics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Synthesis and degradation of fructose 2,6-bisphosphate.

See full target information PFKFB2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 70:78-84 PubMed38836678

2024

M2 macrophages regulate KDM6B/PFKFB2 metabolic reprogramming of cervical squamous cell carcinoma through CXCL1.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Longzhang Huang,Lihua Cao

Functional & integrative genomics 23:322 PubMed37864733

2023

miR-21-5p inhibits the growth of brain glioma cells through regulating the glycolysis mediated by PFKFB2.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Jianmin Liu

Journal of cellular and molecular medicine 27:1384-1397 PubMed37005492

2023

Lin28 promoting the protective effect of PMSCs on hepatic ischaemia-reperfusion injury by regulating glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhou,Junbo Li,Jin Wang,Huifang Yang,Xiaoyun Xie,Zhishui Chen,Bo Zhang

Investigative ophthalmology & visual science 63:2 PubMed36469028

2022

Overexpression of Rhodopsin or Its Mutants Leads to Energy Metabolism Dysfunction in 661w Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Xin Wang,Ruowen Gong,Gezhi Xu,Min Zhu

Science advances 8:eadd3339 PubMed36197977

2022

CARM1-mediated methylation of ASXL2 impairs tumor-suppressive function of MLL3/COMPASS.

Applications

Unspecified application

Species

Unspecified reactive species

Zibo Zhao,Emily Jane Rendleman,Aileen Patricia Szczepanski,Marc Alard Morgan,Lu Wang,Ali Shilatifard

Molecular medicine reports 24: PubMed34109434

2021

Shikonin inhibits the Warburg effect, cell proliferation, invasion and migration by downregulating PFKFB2 expression in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Sha,Zhiqiang Lv,Yujun Liu,Yun Zhang,Xin Sui,Teng Wang,Hui Zhang

OncoTargets and therapy 13:11265-11275 PubMed33173315

2020

miR-1297 Suppresses Osteosarcoma Proliferation and Aerobic Glycolysis by Regulating PFKFB2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Pan,Haibo Li,Jingxue Tan,Xiaokun Weng,Li Zhou,Yiping Weng,Xiaojian Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKFB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB2 antibody (AB234865)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB2 antibody (AB234865)

Western blot - Anti-PFKFB2 antibody (AB234865)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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