JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241506

Anti-PFKFB2 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal PFKFB2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PFKFB2 aa 450 to C-terminus.

View Alternative Names

PFK/FBPase 2, PFKFB2

2 Images
Western blot - Anti-PFKFB2 antibody (AB241506)
  • WB

Supplier Data

Western blot - Anti-PFKFB2 antibody (AB241506)

All lanes:

Western blot - Anti-PFKFB2 antibody (ab241506) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 58 kDa

true

Exposure time: 30s

Immunoprecipitation - Anti-PFKFB2 antibody (AB241506)
  • IP

Supplier Data

Immunoprecipitation - Anti-PFKFB2 antibody (AB241506)

PFKFB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab241506 used for IP at 6 μg per reaction. Western blot was perfomed on the immunoprecipitate using ab241506 at 0.4 μg/ml.

Lane 1 : ab241506 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PFKFB2 antibody (ab241506)

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PFKFB2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60825

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241506 was affinity purified using an epitope specific to PFKFB2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphofructokinase/fructose-26-bisphosphatase 2 often called PFKFB2 plays a significant role in the regulation of glycolysis and gluconeogenesis. This bifunctional enzyme has a molecular mass of approximately 55 kDa. PFKFB2 catalyzes the conversion between fructose-6-phosphate and fructose-26-bisphosphate. It is mainly expressed in cardiac tissue though expression has been noticed in other tissues such as skeletal muscle and liver.
Biological function summary

PFKFB2 regulates the levels of fructose-26-bisphosphate an important metabolic signal molecule. This molecule modulates the activity of key enzymes in glycolysis. PFKFB2 forms part of a complex that controls the glycolytic flux in response to hormonal and nutritional changes. By increasing levels of fructose-26-bisphosphate it strongly activates phosphofructokinase-1 (PFK-1) pushing cells towards glycolytic metabolism under conditions of increased energy demand.

Pathways

PFKFB2 directly influences the glycolysis and gluconeogenesis pathways. The regulation of these pathways by PFKFB2 ties it closely to energy metabolism. Additionally it has functional connections with enzymes such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase) helping to balance the glycolytic and gluconeogenic flux according to cellular needs.

Studies implicate PFKFB2 in conditions such as ischemic heart disease and certain cancers. Ischemic heart disease involves energy metabolism disruption where PFKFB2 plays a role in sustaining cardiac energy supply under oxygen-limited conditions. In cancer PFKFB2's altered expression correlates with the Warburg effect where cancer cells favor glycolysis even in the presence of oxygen. This connection makes PFKFB2 relevant to research fields exploring metabolic interventions in cancer therapeutics.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Synthesis and degradation of fructose 2,6-bisphosphate.
See full target information PFKFB2
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB70175

Anti-PFKFB2 antibody

primary-antibodies

pfkfb2-antibody-ab70175

5

(3 reviews)

Alternative Product
Primary Antibodies

AB234865

Anti-PFKFB2 antibody

primary-antibodies

pfkfb2-antibody-ab234865

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com