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AB70175

Anti-PFKFB2 antibody

5

(3 Reviews)

|

(2 Publications)

Rabbit Polyclonal PFKFB2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFKFB2.

View Alternative Names

PFK/FBPase 2, PFKFB2

2 Images
Western blot - Anti-PFKFB2 antibody (AB70175)
  • WB

Unknown

Western blot - Anti-PFKFB2 antibody (AB70175)

Various other bands are detected, from 26 to over 117kDa.

All lanes:

Western blot - Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 30 µg

Lane 2:

Extracts from Jurkat cells at 30 µg with immunising peptide

Predicted band size: 58 kDa

Observed band size: 58 kDa

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Western blot - Anti-PFKFB2 antibody (AB70175)
  • WB

AbReview21725****

Western blot - Anti-PFKFB2 antibody (AB70175)

Blocking Step : 5% BSA for 1 hour at 22°C

All lanes:

Western blot - Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

All lanes:

Whole cell lysate of mouse NIH 3T£ cells at 25 µg

Secondary

All lanes:

An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution

Predicted band size: 58 kDa

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Exposure time: 8min

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PFKFB2. The exact immunogen used to generate this antibody is proprietary information.

O60825

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphofructokinase/fructose-26-bisphosphatase 2 often called PFKFB2 plays a significant role in the regulation of glycolysis and gluconeogenesis. This bifunctional enzyme has a molecular mass of approximately 55 kDa. PFKFB2 catalyzes the conversion between fructose-6-phosphate and fructose-26-bisphosphate. It is mainly expressed in cardiac tissue though expression has been noticed in other tissues such as skeletal muscle and liver.
Biological function summary

PFKFB2 regulates the levels of fructose-26-bisphosphate an important metabolic signal molecule. This molecule modulates the activity of key enzymes in glycolysis. PFKFB2 forms part of a complex that controls the glycolytic flux in response to hormonal and nutritional changes. By increasing levels of fructose-26-bisphosphate it strongly activates phosphofructokinase-1 (PFK-1) pushing cells towards glycolytic metabolism under conditions of increased energy demand.

Pathways

PFKFB2 directly influences the glycolysis and gluconeogenesis pathways. The regulation of these pathways by PFKFB2 ties it closely to energy metabolism. Additionally it has functional connections with enzymes such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase) helping to balance the glycolytic and gluconeogenic flux according to cellular needs.

Studies implicate PFKFB2 in conditions such as ischemic heart disease and certain cancers. Ischemic heart disease involves energy metabolism disruption where PFKFB2 plays a role in sustaining cardiac energy supply under oxygen-limited conditions. In cancer PFKFB2's altered expression correlates with the Warburg effect where cancer cells favor glycolysis even in the presence of oxygen. This connection makes PFKFB2 relevant to research fields exploring metabolic interventions in cancer therapeutics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Synthesis and degradation of fructose 2,6-bisphosphate.
See full target information PFKFB2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 515:37-43 PubMed31122697

2019

miR-613 inhibits Warburg effect in gastric cancer by targeting PFKFB2.

Applications

Unspecified application

Species

Unspecified reactive species

Haipeng Liu,Kang Chen,Li Wang,Xiangting Zeng,Zeping Huang,Mei Li,Peng Dong,Xiao Chen

JCI insight 1: PubMed27398409

2016

Fibulin-1 regulates the pathogenesis of tissue remodeling in respiratory diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Marion A Cooley,Andrew G Jarnicki,Alan C-Y Hsu,Prema M Nair,Tatt Jhong Haw,Michael Fricker,Shaan L Gellatly,Richard Y Kim,Mark D Inman,Gavin Tjin,Peter A B Wark,Marjorie M Walker,Jay C Horvat,Brian G Oliver,W Scott Argraves,Darryl A Knight,Janette K Burgess,Philip M Hansbro
View all publications
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