Rabbit Recombinant Monoclonal PFKFB2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-PFKFB2 antibody [EPR19875-136] - BSA and Azide free (Capture) (AB242607), expandable thumbnail](https://content.abcam.com/products/images/pfkfb2-antibody-epr19875-136-bsa-and-azide-free-capture-ab242607--sandwich-elisa-img175811.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-PFKFB2 antibody [EPR19875-136] - BSA and Azide free (Capture) (AB242607)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Synthesis and degradation of fructose 2,6-bisphosphate.
Alternative names
PFK/FBPase 2, PFKFB2
Recommended products
Rabbit Recombinant Monoclonal PFKFB2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
PFK/FBPase 2, PFKFB2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19875-136
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab242607 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PFKFB2. The recommended pair for sandwich ELISA is:
Capture: ab242607, Human PFKFB2 Capture Antibody (unconjugated)
Detector: Anti-PFKFB2 antibody [EPR19875-44] - BSA and Azide free (Detector) ab242853, Human PFKFB2 Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
Phosphofructokinase/fructose-26-bisphosphatase 2 often called PFKFB2 plays a significant role in the regulation of glycolysis and gluconeogenesis. This bifunctional enzyme has a molecular mass of approximately 55 kDa. PFKFB2 catalyzes the conversion between fructose-6-phosphate and fructose-26-bisphosphate. It is mainly expressed in cardiac tissue though expression has been noticed in other tissues such as skeletal muscle and liver.
- Biological function summary
PFKFB2 regulates the levels of fructose-26-bisphosphate an important metabolic signal molecule. This molecule modulates the activity of key enzymes in glycolysis. PFKFB2 forms part of a complex that controls the glycolytic flux in response to hormonal and nutritional changes. By increasing levels of fructose-26-bisphosphate it strongly activates phosphofructokinase-1 (PFK-1) pushing cells towards glycolytic metabolism under conditions of increased energy demand.
- Pathways
PFKFB2 directly influences the glycolysis and gluconeogenesis pathways. The regulation of these pathways by PFKFB2 ties it closely to energy metabolism. Additionally it has functional connections with enzymes such as phosphofructokinase-1 (PFK-1) and fructose-16-bisphosphatase (FBPase) helping to balance the glycolytic and gluconeogenic flux according to cellular needs.
- Associated diseases and disorders
Studies implicate PFKFB2 in conditions such as ischemic heart disease and certain cancers. Ischemic heart disease involves energy metabolism disruption where PFKFB2 plays a role in sustaining cardiac energy supply under oxygen-limited conditions. In cancer PFKFB2's altered expression correlates with the Warburg effect where cancer cells favor glycolysis even in the presence of oxygen. This connection makes PFKFB2 relevant to research fields exploring metabolic interventions in cancer therapeutics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-PFKFB2 antibody [EPR19875-136] - BSA and Azide free (Capture) (ab242607), expandable thumbnail](https://content.abcam.com/products/images/pfkfb2-antibody-epr19875-136-bsa-and-azide-free-capture-ab242607--sandwich-elisa-img175811.jpg "Sandwich ELISA - Anti-PFKFB2 antibody [EPR19875-136] - BSA and Azide free (Capture) (ab242607)")
Sandwich ELISA - Anti-PFKFB2 antibody [EPR19875-136] - BSA and Azide free (Capture) (ab242607)
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214574).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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