Anti-PFKFB3 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB3 antibody (AB96699)

Immunohistochemical analysis of paraffin-embedded bt483 xenograft tissue labelling PFKFB3 protein at nucleus with ab96699 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

• IP

Unknown

Immunoprecipitation - Anti-PFKFB3 antibody (AB96699)

PFKFB3 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to PFKFB3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96699.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 70kDa : PFKFB3.

All lanes:

Immunoprecipitation - Anti-PFKFB3 antibody (ab96699)

Predicted band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-PFKFB3 antibody (AB96699)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PFKFB3 antibody (ab96699) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 60 kDa

false