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AB326695

Anti-PFKFB3 antibody [SR3683]

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Rabbit Recombinant Monoclonal PFKFB3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

PFK/FBPase 3, Renal carcinoma antigen NY-REN-56, iPFK-2, PFKFB3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB3 antibody [SR3683] (AB326695)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB3 antibody [SR3683] (AB326695)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PFKFB3 using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PFKFB3 antibody [SR3683] (AB326695)
  • WB

Supplier Data

Western blot - Anti-PFKFB3 antibody [SR3683] (AB326695)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : JK whole cell lysates
Lane 2 : K562 whole cell lysates
Lane 3 : PC12 whole cell lysates
Lane 4 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 60 kDa
Observed band size : 60 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-PFKFB3 antibody [SR3683] (ab326695) at 1/1000 dilution

Lane 1:

JK whole cell lysates at 20 µg

Lane 2:

K562 whole cell lysates at 20 µg

Lane 3:

PC12 whole cell lysates at 20 µg

Lane 4:

Mouse spleen lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Exposure time: 4s

Western blot - Anti-PFKFB3 antibody [SR3683] (AB326695)
  • WB

Supplier Data

Western blot - Anti-PFKFB3 antibody [SR3683] (AB326695)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : PFKFB3 Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-PFKFB3 antibody [SR3683] (ab326695) at 1/1000 dilution

Lane 1:

Wild-type HT-1080 cell lysate at 20 µg

Lane 2:

PFKFB3 Rabbit mAb knockdown HT-1080 cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR3683

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate.
See full target information PFKFB3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com