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Rabbit Polyclonal PFKFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (AB137785), expandable thumbnail
  • Western blot - Anti-PFKFB4 antibody (AB137785), expandable thumbnail
  • Western blot - Anti-PFKFB4 antibody (AB137785), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79.97% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16877

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Tested
Expected
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Synthesis and degradation of fructose 2,6-bisphosphate.

Alternative names

Recommended products

Rabbit Polyclonal PFKFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16877
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.

Biological function summary

PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.

Pathways

PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.

Associated diseases and disorders

PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with a citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (ab137785)

    Immunocytochemistry/ Immunofluorescence of NT2D1 staining PFKFB4 using ab137785 at a dilution of 1/500. Nuclear staining using Hoechst 33342.

  • Western blot - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Western blot - Anti-PFKFB4 antibody (ab137785)

    All lanes: Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution

    All lanes: Rat tissue extract at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 54 kDa

  • Western blot - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Western blot - Anti-PFKFB4 antibody (ab137785)

    10% SDS PAGE

    All lanes: Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution

    All lanes: Raji whole cell lysate at 30 µg

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (ab137785)

    Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

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Product protocols

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