Rabbit Polyclonal PFKFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79.97% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Expected | Tested |
Rat | Tested | Expected | Expected |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Select an associated product type
Synthesis and degradation of fructose 2,6-bisphosphate.
PFK/FBPase 4, PFKFB4
Rabbit Polyclonal PFKFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79.97% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.
PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.
PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.
PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with a citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence of NT2D1 staining PFKFB4 using ab137785 at a dilution of 1/500. Nuclear staining using Hoechst 33342.
All lanes: Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution
All lanes: Rat tissue extract at 50 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 54 kDa
10% SDS PAGE
All lanes: Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 54 kDa
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min
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