AB137785

Anti-PFKFB4 antibody

Name: Anti-PFKFB4 antibody (ab137785) | Abcam
Brand: Abcam Limited
SKU: AB137785
Price: 525 USD
Availability: InStock

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(22 Publications)

Rabbit Polyclonal PFKFB4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKFB4 aa 200-500.

View Alternative Names

PFK/FBPase 4, PFKFB4

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (AB137785)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (AB137785)

Immunocytochemistry/ Immunofluorescence of NT2D1 staining PFKFB4 using ab137785 at a dilution of 1/500. Nuclear staining using Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with a citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

Unknown

Western blot - Anti-PFKFB4 antibody (AB137785)

10% SDS PAGE

All lanes:

Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-PFKFB4 antibody (AB137785)

All lanes:

Western blot - Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution

All lanes:

Rat tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PFKFB4 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

Q16877

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.

Biological function summary

PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.

Pathways

PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.

PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Synthesis and degradation of fructose 2,6-bisphosphate.

See full target information PFKFB4

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 53: PubMed40116122

2025

PFKFB4 promotes endometrial cancer by regulating glycolysis through SRC‑3 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Wu,Jianzhen Zhao,Shuangshuang Zhao,Jianfang Li,Jin Luo,Yingmei Wang

Cellular and molecular biology (Noisy-le-Grand, France) 70:73-78 PubMed39097893

2024

MiR-3195 inhibits non-small cell lung cancer malignant behaviors along with cisplatin resistance through targeting PFKFB4.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Chen,Xiaoyan Gao,Yahui Shen

Cancer cell international 23:257 PubMed37919747

2023

Comparative clinical significance and biological roles of PFKFB family members in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Fang Hu,Chih-Wen Shu,Cheng-Hsin Lee,Ching-Jiunn Tseng,Yu-Hsiang Chou,Pei-Feng Liu

Scientific reports 13:16284 PubMed37770581

2023

Differential roles of highly expressed PFKFB4 in colon adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Gu,Xingchen Dai,Yongli Huang,Yuhuan Zhang,Lintao Dong,Chanchan Gao,Fang Wang

Cell death & disease 14:326 PubMed37179372

2023

Hypoxia-mediated promotion of glucose metabolism in non-small cell lung cancer correlates with activation of the EZH2/FBXL7/PFKFB4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhou,Yang Lin,Xiuhua Kang,Zhicheng Liu,Juntao Zou,Fei Xu

Cellular and molecular gastroenterology and hepatology 15:1325-1350 PubMed36806581

2023

PFKFB4 Drives the Oncogenicity in TP53-Mutated Hepatocellular Carcinoma in a Phosphatase-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Shing Kam,Daniel Wai-Hung Ho,Vanessa Sheung-In Ming,Lu Tian,Karen Man-Fong Sze,Vanilla Xin Zhang,Yu-Man Tsui,Abdullah Husain,Joyce Man-Fong Lee,Carmen Chak-Lui Wong,Albert Chi-Yan Chan,Tan-To Cheung,Lo-Kong Chan,Irene Oi-Lin Ng

Cell reports 41:111756 PubMed36476868

2022

Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Dai,Spencer R Rosario,Eriko Katsuta,Abhisha Sawant Dessai,Emily J Paterson,Aaron T Novickis,Eduardo Cortes Gomez,Bokai Zhu,Song Liu,Hai Wang,Scott I Abrams,Mukund Seshadri,Wiam Bshara,Subhamoy Dasgupta

Clinical and translational medicine 12:e999 PubMed35917405

2022

Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yimei Lai,Siyuan Zhao,Binfeng Chen,Yuefang Huang,Chaohuan Guo,Mengyuan Li,Baokui Ye,Shuyi Wang,Hui Zhang,Niansheng Yang

International journal of general medicine 15:6341-6353 PubMed35942289

2022

Effect of PFKFB4 on the Prognosis and Immune Regulation of NSCLC and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhou,Yongfei Fan,Binzhe Qiu,Ming Lou,Xiaoshuang Liu,Kai Yuan,Jichun Tong

BMC cancer 22:562 PubMed35590288

2022

KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Bin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PFKFB4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PFKFB4 antibody (AB137785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB4 antibody (AB137785)

Western blot - Anti-PFKFB4 antibody (AB137785)

Western blot - Anti-PFKFB4 antibody (AB137785)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise