Rabbit Polyclonal PFKL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFKL aa 1-50.
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Publications
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- Neoplasma 69:1041-10532022Exosomes from M2 macrophages promoted glycolysis in FaDu cells by inhibiting PDLIM2 expression to stabilize PFKL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Wang,Guang-Yao Li,Lei Zhou,Huai-Li Jiang,Yue Yang,Hai-Tao WuPubMed 35723199
- Cell death & disease 12:4082021The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Gao,Mengqiu Huang,Xi Chen,Jianping Chen,Zhiwei Zou,Linlin Li,Kaiyuan Ji,Zhirui Nie,Bingsheng Yang,Zibo Wei,Pengfei Xu,Junshuang Jia,Qianbing Zhang,Hongfen Shen,Qianli Wang,Keyi Li,Lingqun Zhu,Meng Wang,Shuangyan Ye,Sisi Zeng,Ying Lin,Zhili Rong,Yang Xu,Peng Zhu,Hui Zhang,Bingtao Hao,Qiuzhen LiuPubMed 33859186
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PFKL aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P17858
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).
Alternative names
ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL
Recommended products
Rabbit Polyclonal PFKL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFKL aa 1-50.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PFKL aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P17858
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PFKL also known as phosphofructokinase liver type is an important enzyme in glycolysis. It catalyzes the conversion of fructose-6-phosphate to fructose-16-bisphosphate. This enzyme plays a significant role in regulating the glycolytic flux due to its allosteric nature. Weighing approximately 85 kDa PFKL is found abundantly in liver tissues but you can also find it in various other tissues such as muscle and brain. Its expression adapts according to the metabolic demand reflecting its importance in energy homeostasis.
- Biological function summary
Phosphofructokinase liver type is an integral component of cellular energy production. It functions in the cytoplasmic compartment and often operates as a part of a tetrameric complex. The enzyme activity is subject to regulation by several metabolites including ATP ADP and citrate which modulate its activity for efficient energy use. It acts as a metabolic checkpoint influencing the rate of glucose breakdown in response to cellular energy needs.
- Pathways
Phosphofructokinase liver type plays a central role in the glycolytic pathway. This pathway is critical for ATP generation especially in conditions where cells rely heavily on glucose. It cooperates with other enzymes like hexokinase and pyruvate kinase to ensure a smooth conversion of glucose to pyruvate facilitating subsequent processes like the citric acid cycle. By integrating signals from multiple pathways it serves as a nodal point for metabolic control affecting various physiological and biochemical processes.
- Associated diseases and disorders
Dysregulation of phosphofructokinase liver type can lead to metabolic diseases such as type 2 diabetes and cancer. Altered activity or expression of PFKL influences glucose metabolism potentially resulting in insulin resistance or enhanced tumor glycolysis respectively. Moreover PFKL shares connections with other proteins like insulin receptor substrates in diabetes and oncogenes in cancer influencing disease progression and response to therapies. Understanding PFKL's function and regulation can aid in developing strategies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PFKL antibody (ab241093)
Lysates prepared in NETN lysis buffer.
All lanes: Western blot - Anti-PFKL antibody (ab241093) at 0.4 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3min
Immunoprecipitation - Anti-PFKL antibody (ab241093)
PFKL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241093 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241093 at 1 μg/ml.
Lane 1: ab241093 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-PFKL antibody (ab241093)
Predicted band size: 85 kDa
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