AB241093

Anti-PFKL antibody

Name: Anti-PFKL antibody (ab241093) | Abcam
Brand: Abcam Limited
SKU: AB241093

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(3 Publications)

Rabbit Polyclonal PFKL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PFKL aa 1-50.

View Alternative Names

ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL

2 Images

Supplier Data

Immunoprecipitation - Anti-PFKL antibody (AB241093)

PFKL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241093 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241093 at 1 μg/ml.

Lane 1 : ab241093 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-PFKL antibody (ab241093)

Predicted band size: 85 kDa

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Supplier Data

Western blot - Anti-PFKL antibody (AB241093)

Lysates prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-PFKL antibody (ab241093) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 85 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PFKL aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P17858

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKL also known as phosphofructokinase liver type is an important enzyme in glycolysis. It catalyzes the conversion of fructose-6-phosphate to fructose-16-bisphosphate. This enzyme plays a significant role in regulating the glycolytic flux due to its allosteric nature. Weighing approximately 85 kDa PFKL is found abundantly in liver tissues but you can also find it in various other tissues such as muscle and brain. Its expression adapts according to the metabolic demand reflecting its importance in energy homeostasis.

Biological function summary

Phosphofructokinase liver type is an integral component of cellular energy production. It functions in the cytoplasmic compartment and often operates as a part of a tetrameric complex. The enzyme activity is subject to regulation by several metabolites including ATP ADP and citrate which modulate its activity for efficient energy use. It acts as a metabolic checkpoint influencing the rate of glucose breakdown in response to cellular energy needs.

Pathways

Phosphofructokinase liver type plays a central role in the glycolytic pathway. This pathway is critical for ATP generation especially in conditions where cells rely heavily on glucose. It cooperates with other enzymes like hexokinase and pyruvate kinase to ensure a smooth conversion of glucose to pyruvate facilitating subsequent processes like the citric acid cycle. By integrating signals from multiple pathways it serves as a nodal point for metabolic control affecting various physiological and biochemical processes.

Dysregulation of phosphofructokinase liver type can lead to metabolic diseases such as type 2 diabetes and cancer. Altered activity or expression of PFKL influences glucose metabolism potentially resulting in insulin resistance or enhanced tumor glycolysis respectively. Moreover PFKL shares connections with other proteins like insulin receptor substrates in diabetes and oncogenes in cancer influencing disease progression and response to therapies. Understanding PFKL's function and regulation can aid in developing strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed : 22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).

See full target information PFKL

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf024 PubMed40918646

2025

Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Dopler,Edwin S Kyei-Baffour,Mandy Kerkhoff,Ferhat Alkan,Yuval Malka,Kelly Hoefakker,Rob van der Kammen,Liesbeth Hoekman,Onno Bleijerveld,Antonia Bradaric,Maarten Altelaar,Jonathan W Yewdell,Pia Kvistborg,William J Faller

Neoplasma 69:1041-1053 PubMed35723199

2022

Exosomes from M2 macrophages promoted glycolysis in FaDu cells by inhibiting PDLIM2 expression to stabilize PFKL.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Guang-Yao Li,Lei Zhou,Huai-Li Jiang,Yue Yang,Hai-Tao Wu

Cell death & disease 12:408 PubMed33859186

2021

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Gao,Mengqiu Huang,Xi Chen,Jianping Chen,Zhiwei Zou,Linlin Li,Kaiyuan Ji,Zhirui Nie,Bingsheng Yang,Zibo Wei,Pengfei Xu,Junshuang Jia,Qianbing Zhang,Hongfen Shen,Qianli Wang,Keyi Li,Lingqun Zhu,Meng Wang,Shuangyan Ye,Sisi Zeng,Ying Lin,Zhili Rong,Yang Xu,Peng Zhu,Hui Zhang,Bingtao Hao,Qiuzhen Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKL antibody

Immunoprecipitation - Anti-PFKL antibody (AB241093)

Western blot - Anti-PFKL antibody (AB241093)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise