Name: Anti-PFKM antibody [EPR10734(B)]
SKU: ab154804
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal PFKM antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Pig, Human samples. Cited in 30 publications.

Images

Western blot - Anti-PFKM antibody [EPR10734(B)] (AB154804), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Expected
Rat	Tested	Expected	Expected
Pig	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Pig	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/300	Notes For unpurified use at 1: 50 - 1:100.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Pig	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/500	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Pig	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Product

Target data

See full target information PFKM

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Alternative names

PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A

Recommended products

Rabbit Recombinant Monoclonal PFKM antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Pig, Human samples. Cited in 30 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10734(B)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PFKM also known as phosphofructokinase muscle type is a protein involved in glycolysis. It functions as an enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate. This reaction is important for the regulation of glucose metabolism. The PFKM protein has an approximate mass of 85 kDa and is mainly expressed in muscle tissues. Alternate names for PFKM include 6-phosphofructokinase type M and PFK-1 highlighting its critical role in glycolytic processes.

Biological function summary

PFKM acts as an important regulatory component in glycolysis because it controls the rate-limiting step of this metabolic pathway. It forms a homo-tetramer complex which can vary in its exact subunit composition to modulate activity based on cellular energy requirements. The presence of allosteric sites on PFKM allows it to respond to different metabolic signals such as ATP and AMP levels highlighting its role in energy homeostasis. The ability to alter its conformation and enzymatic activity enables PFKM to efficiently regulate energy production and consumption at the cellular level.

Pathways

PFKM plays a pivotal role in the glycolytic pathway facilitating the breakdown of glucose for energy production. This enzyme not only relates to the glycolysis pathway but also interacts with other key proteins such as hexokinase and pyruvate kinase forming a tightly controlled network for efficient metabolic flow. PFKM's activity influences downstream processes including the citric acid cycle and oxidative phosphorylation through its provision of pyruvate.

Associated diseases and disorders

Defects in PFKM are associated with glycogen storage disease type VII also known as Tarui's disease. This disorder results from a deficiency in PFKM activity leading to the accumulation of glycogen in muscle tissues and resulting in exercise intolerance. Additionally PFKM dysfunction has connections to cancer given that many cancer cells exhibit increased glycolytic activity. Considering its role in disease studying PFKM can provide insights into potential therapeutic targets for conditions characterized by metabolic abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 seconds; Lane 2: 10 seconds; Lane 3: 180 seconds.
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/1000 dilution
Lane 1: DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)
Lane 2: Western blot - Recombinant Human PFKP protein (Recombinant Human PFKP protein ab132823)
Lane 3: GST tagged Recombinant Human PFKL protein (Full length, 116 KDa)
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/10000 dilution
All lanes: Pig heart lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Flow Cytometry (Intracellular) - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1/300 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/2000 dilution
Lane 1: Rat skeletal muscle lysates at 15 µg
Lane 2: Mouse skeletal muscle lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa, 85 kDa
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/2000 dilution
All lanes: Human fetal heart lysates at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 85 kDa
This image is courtesy of a customer review submitted by Eric England
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Western blot analysis of Pig skeletal muscle tissue lysate using ab154804 (1:2000 in 1x PBS + 0.1% Tween-20 for 1 hour at 22°C) to label PFKM. A IRDye® 680-conjugated Donkey anti-rabbit IgG (1:40000) was used as the secondary antibody. The image shows increasing amounts of loaded protein for antibody optimization. The far left lane is the molecular weight standard.
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 81 kDa
Exposure time: 5min
Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804)
All lanes: Western blot - Anti-PFKM antibody [EPR10734(B)] (ab154804) at 1/1000 dilution
Lane 1: Fetal brain lysate at 10 µg
Lane 2: Fetal heart lysate at 10 µg
Lane 3: Ramos lysate at 10 µg
Lane 4: SHSY5Y lysate at 10 µg
Lane 5: HeLa lysate at 10 µg
Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.
Flow Cytometry (Intracellular) - Anti-PFKM antibody [EPR10734(B)] (ab154804)
Intracellular flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).