Rabbit Recombinant Monoclonal PFKM antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human, Mouse, Recombinant full length protein - Human samples. Cited in 18 publications.
Images
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img176478.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)")
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img126205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)")
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img116821.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)")
![Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (AB181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--immunocytochemistry-immunofluorescence-img93082.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)")
![Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (AB181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--flow-cytometry-intracellular-img78161.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)")
Publications
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- Pathogens and global health 117:409-4162022protoscoleces enhance glycolysis to promote M2 Macrophages through PI3K/Akt/mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Yaogang Zhang,Zihan Yang,Yuan Jiang,Li Sun,Dengliang Huang,Meiyuan Tian,Yinhong Shen,Jun Deng,Jing Hou,Yanyan MaPubMed 35876088
- Acta biochimica et biophysica Sinica 54:625-6362022Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieya Wei,Yueyi Yang,Daimo Guo,Siqun Xu,Hongcan Huang,Demao Zhang,Jing Xie,Xuedong ZhouPubMed 35593470
- Cancer discovery 12:1718-17412022IFNα Potentiates Anti-PD-1 Efficacy by Remodeling Glucose Metabolism in the Hepatocellular Carcinoma Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Hu,Mincheng Yu,Xiaolu Ma,Jialei Sun,Chenglong Liu,Chunyan Wang,Suiyi Wu,Peiyao Fu,Zhen Yang,Yungang He,Yuanyuan Zhu,Cheng Huang,Xinrong Yang,Yinghong Shi,Shuangjian Qiu,Huichuan Sun,Andrew X Zhu,Jian Zhou,Yang Xu,Di Zhu,Jia FanPubMed 35412588
- Stem cells international 2022:43636322022Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Qin Li,Hui-Jie Jiang,Xiu-Yun Su,Li Feng,Na-Zhi Zhan,Shan-Shan Li,Zi-Jie Chen,Bo-Han Chang,Peng-Zhen Cheng,Liu Yang,Guo-Xian PeiPubMed 35069747
- Scientific reports 11:235802021Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Perico,Marina Morigi,Anna Pezzotta,Daniela Corna,Valerio Brizi,Sara Conti,Cristina Zanchi,Fabio Sangalli,Piera Trionfini,Sara Buttò,Christodoulos Xinaris,Susanna Tomasoni,Carlamaria Zoja,Giuseppe Remuzzi,Ariela Benigni,Barbara ImbertiPubMed 34880332
- Cancer cell international 21:6192021Antitumor effects of the small molecule DMAMCL in neuroblastoma via suppressing aerobic glycolysis and targeting PFKL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimeng Zhang,Zhongyan Hua,Gen Ba,Ning Xu,Jianing Miao,Guifeng Zhao,Wei Gong,Zhihui Liu,Carol J Thiele,Zhijie LiPubMed 34819091
- Biochemistry and cell biology = Biochimie et biologie cellulaire 100:75-842021PDGFRβ modulates aerobic glycolysis in osteosarcoma HOS cells via the PI3K/AKT/mTOR/c-Myc pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu-Ying Tang,Jia-Qi Guo,Bo-Tao Sang,Jun-Ning Cheng,Xiang-Mei WuPubMed 34678088
- The journal of obstetrics and gynaecology research 47:3913-39222021Downregulation of GTSE1 leads to the inhibition of proliferation, migration, and Warburg effect in cervical cancer by blocking LHDA expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLongyi Chen,Youwen Zhong,Xiuwei Yang,Qingyue Zhang,Xiaoling WuPubMed 34482592
- Cell 184:4480-4494.e152021Selective activation of PFKL suppresses the phagocytic oxidative burst.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeri Amara,Madison P Cooper,Maria A Voronkova,Bradley A Webb,Eric M Lynch,Justin M Kollman,Taylur Ma,Kebing Yu,Zijuan Lai,Dewakar Sangaraju,Nobuhiko Kayagaki,Kim Newton,Matthew Bogyo,Steven T Staben,Vishva M DixitPubMed 34320407
- Frontiers in cell and developmental biology 9:6778602021Inhibition of miR-185-3p Confers Erlotinib Resistance Through Upregulation of PFKL/MET in Lung Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Li,Xinling Zhu,Conghu YuanPubMed 34368128
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Additional Targets
PFKL
Alternative names
PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A
Recommended products
Rabbit Recombinant Monoclonal PFKM antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human, Mouse, Recombinant full length protein - Human samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11904
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PFKM and PFKL are key isoforms of phosphofructokinase an enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate in glycolysis. PFKM also known as phosphofructokinase muscle type has a mass of approximately 85 kDa while PFKL or phosphofructokinase liver type is about 83 kDa. These isoforms express mainly in muscle and liver tissues respectively where they play a significant role in energy metabolism.
- Biological function summary
PFKM and PFKL are important components of the glycolysis metabolic pathway. These enzymes form a multi-subunit complex called phosphofructokinase that regulates the rate-limiting step of glycolysis ensuring efficient energy production. This complex adapts to changes in cellular energy demands and environmental conditions therefore controlling glucose breakdown.
- Pathways
PFKM and PFKL are integral to glycolysis and gluconeogenesis processes essential for energy production and glucose homeostasis. PFKM closely interacts with hexokinase and pyruvate kinase in the glycolytic pathway ensuring continuation of the energy yield process. PFKL participates similarly with both aiding in maintaining the proper functioning of cellular metabolic activities.
- Associated diseases and disorders
Abnormalities in PFKM and PFKL activity associate with glycogen storage disease type VII (Tarui's disease) and non-insulin-dependent diabetes mellitus. PFKM mutations lead to impaired glycolysis in muscle cells causing exercise intolerance and muscle cramps while PFKL variants can alter glucose metabolism contributing to diabetes development. These proteins' roles in diseases provide insight into potential therapeutic targets for metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img176478.png "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)
PFKM + PFKL Western blot staining using rabbit Anti-PFKM + PFKL antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution
Lane 1: GST tagged Recombinant Human PFKL protein (Full length, 116 KDa)
Lane 2: DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 180s
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img126205.jpg "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)
All lanes: Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/5000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Observed band size: 78 kDa
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--western-blot-img116821.jpg "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)
All lanes: Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: Raw264.7 cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution
Observed band size: 78 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (ab181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--immunocytochemistry-immunofluorescence-img93082.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
![Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (ab181064), expandable thumbnail](https://content.abcam.com/products/images/pfkm-pfkl-antibody-epr11904-ab181064--flow-cytometry-intracellular-img78161.jpg "Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedC6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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