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AB181064

Anti-PFKM + PFKL antibody [EPR11904]

5

(1 Review)

|

(26 Publications)

Rabbit Recombinant Monoclonal PFKM antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human, Mouse, Recombinant full length protein - Human samples. Cited in 26 publications.

View Alternative Names

PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A, ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedC6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)
  • WB

Supplier Data

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

All lanes:

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

Observed band size: 78 kDa

false

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)
  • WB

Supplier Data

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

All lanes:

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Observed band size: 78 kDa

false

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)
  • WB

Lab

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution

Lane 1:

GST tagged Recombinant Human PFKL protein (Full length, 116 KDa)

Lane 2:

DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

  • Carrier free

    Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free

  • 578 PE

    PE Anti-PFKM + PFKL antibody [EPR11904]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PFKM + PFKL antibody [EPR11904]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PFKM + PFKL antibody [EPR11904]

  • 660 APC

    APC Anti-PFKM + PFKL antibody [EPR11904]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PFKM + PFKL antibody [EPR11904]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PFKM + PFKL antibody [EPR11904]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11904

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Complementary Products

Assay Kits

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB204131

Anti-PFKM + PFKP antibody [EPR17314]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKM and PFKL are key isoforms of phosphofructokinase an enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate in glycolysis. PFKM also known as phosphofructokinase muscle type has a mass of approximately 85 kDa while PFKL or phosphofructokinase liver type is about 83 kDa. These isoforms express mainly in muscle and liver tissues respectively where they play a significant role in energy metabolism.
Biological function summary

PFKM and PFKL are important components of the glycolysis metabolic pathway. These enzymes form a multi-subunit complex called phosphofructokinase that regulates the rate-limiting step of glycolysis ensuring efficient energy production. This complex adapts to changes in cellular energy demands and environmental conditions therefore controlling glucose breakdown.

Pathways

PFKM and PFKL are integral to glycolysis and gluconeogenesis processes essential for energy production and glucose homeostasis. PFKM closely interacts with hexokinase and pyruvate kinase in the glycolytic pathway ensuring continuation of the energy yield process. PFKL participates similarly with both aiding in maintaining the proper functioning of cellular metabolic activities.

Abnormalities in PFKM and PFKL activity associate with glycogen storage disease type VII (Tarui's disease) and non-insulin-dependent diabetes mellitus. PFKM mutations lead to impaired glycolysis in muscle cells causing exercise intolerance and muscle cramps while PFKL variants can alter glucose metabolism contributing to diabetes development. These proteins' roles in diseases provide insight into potential therapeutic targets for metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
See full target information PFKM

Additional targets

PFKL
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:187 PubMed40898360

2025

Identification and validation of a tear fluid-derived protein biomarker signature in patients with amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lena-Sophie Scholl,Antonia F Demleitner,Jenny Riedel,Seren Adachi,Lisa Neuenroth,Clara Meijs,Laura Tzeplaeff,Lucas Caldi Gomes,Ana Galhoz,Isabell Cordts,Christof Lenz,Michael Menden,Paul Lingor

Scientific reports 15:13273 PubMed40246883

2025

Fragile X messenger ribonucleoprotein 1 (FMRP) regulates glycolytic gene expression under chronic hypoxia in HCT116 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Ogura,Xiaoning Sun,Zaijun Zhang,Kentaro Kawata,Jinyu Wu,Ryuma Matsubara,Atsuko Nakanishi Ozeki,Kenzui Taniue,Rena Onoguchi-Mizutani,Shungo Adachi,Koh Nakayama,Nobuhito Goda,Nobuyoshi Akimitsu

Nature communications 15:7323 PubMed39183237

2024

Structural basis for allosteric regulation of human phosphofructokinase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Eric M Lynch,Heather Hansen,Lauren Salay,Madison Cooper,Stepan Timr,Justin M Kollman,Bradley A Webb

Clinical and molecular hepatology 30:771-792 PubMed38915206

2024

UBE2S promotes glycolysis in hepatocellular carcinoma by enhancing E3 enzyme-independent polyubiquitination of VHL.

Applications

Unspecified application

Species

Unspecified reactive species

Renyu Zhang,Can Li,Shuai Zhang,Lingmin Kong,Zekun Liu,Yixiao Guo,Ying Sun,Cong Zhang,Yule Yong,Jianjun Lv,Meng Lu,Man Liu,Dong Wu,Tianjiao Zhang,Haijiao Yang,Ding Wei,Zhinan Chen,Huijie Bian

Cell death & disease 15:354 PubMed38773094

2024

Energy stress-induced circDDX21 promotes glycolysis and facilitates hepatocellular carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Luo,Yang Yang,Guang Zhang,Debao Fang,Kaiyue Liu,Yide Mei,Fang Wang

Cancer research communications 3:2211-2220 PubMed37861293

2023

Antitumor Effects of PRMT5 Inhibition in Sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Verbeke,Aurélien Bourdon,Jean-Philippe Guegan,Laura Leroy,Vanessa Chaire,Elodie Richard,Alban Bessede,Antoine Italiano

The Biochemical journal 480:1411-1427 PubMed37622331

2023

Cancer-associated somatic mutations in human phosphofructokinase-1 reveal a critical electrostatic interaction for allosteric regulation of enzyme activity.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Voronkova,Heather L Hansen,Madison P Cooper,Jacob Miller,Narayanasami Sukumar,Werner J Geldenhuys,Aaron R Robart,Bradley A Webb

Pathogens and global health 117:409-416 PubMed35876088

2022

protoscoleces enhance glycolysis to promote M2 Macrophages through PI3K/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Yaogang Zhang,Zihan Yang,Yuan Jiang,Li Sun,Dengliang Huang,Meiyuan Tian,Yinhong Shen,Jun Deng,Jing Hou,Yanyan Ma

Acta biochimica et biophysica Sinica 54:625-636 PubMed35593470

2022

Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication.

Applications

Unspecified application

Species

Unspecified reactive species

Jieya Wei,Yueyi Yang,Daimo Guo,Siqun Xu,Hongcan Huang,Demao Zhang,Jing Xie,Xuedong Zhou

Cancer discovery 12:1718-1741 PubMed35412588

2022

IFNα Potentiates Anti-PD-1 Efficacy by Remodeling Glucose Metabolism in the Hepatocellular Carcinoma Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hu,Mincheng Yu,Xiaolu Ma,Jialei Sun,Chenglong Liu,Chunyan Wang,Suiyi Wu,Peiyao Fu,Zhen Yang,Yungang He,Yuanyuan Zhu,Cheng Huang,Xinrong Yang,Yinghong Shi,Shuangjian Qiu,Huichuan Sun,Andrew X Zhu,Jian Zhou,Yang Xu,Di Zhu,Jia Fan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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