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AB240237

Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal PFKM antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181064).

Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed C6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181064).

Western blot - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)
  • WB

Lab

Western blot - Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free (AB240237)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PFKM + PFKL antibody [EPR11904] (<a href='/en-us/products/primary-antibodies/pfkm-pfkl-antibody-epr11904-ab181064'>ab181064</a>) at 1/1000 dilution

Lane 1:

GST tagged Recombinant Human PFKL protein (Full length, 116 KDa)

Lane 2:

DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

  • Unconjugated

    Anti-PFKM + PFKL antibody [EPR11904]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PFKM + PFKL antibody [EPR11904]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PFKM + PFKL antibody [EPR11904]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PFKM + PFKL antibody [EPR11904]

  • 578 PE

    PE Anti-PFKM + PFKL antibody [EPR11904]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PFKM + PFKL antibody [EPR11904]

  • 660 APC

    APC Anti-PFKM + PFKL antibody [EPR11904]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11904

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab240237 is the carrier-free version of ab181064.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKM and PFKL are key isoforms of phosphofructokinase an enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate in glycolysis. PFKM also known as phosphofructokinase muscle type has a mass of approximately 85 kDa while PFKL or phosphofructokinase liver type is about 83 kDa. These isoforms express mainly in muscle and liver tissues respectively where they play a significant role in energy metabolism.
Biological function summary

PFKM and PFKL are important components of the glycolysis metabolic pathway. These enzymes form a multi-subunit complex called phosphofructokinase that regulates the rate-limiting step of glycolysis ensuring efficient energy production. This complex adapts to changes in cellular energy demands and environmental conditions therefore controlling glucose breakdown.

Pathways

PFKM and PFKL are integral to glycolysis and gluconeogenesis processes essential for energy production and glucose homeostasis. PFKM closely interacts with hexokinase and pyruvate kinase in the glycolytic pathway ensuring continuation of the energy yield process. PFKL participates similarly with both aiding in maintaining the proper functioning of cellular metabolic activities.

Abnormalities in PFKM and PFKL activity associate with glycogen storage disease type VII (Tarui's disease) and non-insulin-dependent diabetes mellitus. PFKM mutations lead to impaired glycolysis in muscle cells causing exercise intolerance and muscle cramps while PFKL variants can alter glucose metabolism contributing to diabetes development. These proteins' roles in diseases provide insight into potential therapeutic targets for metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
See full target information PFKM

Additional targets

PFKL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39457558

2024

Tetraspanins, GLAST and L1CAM Quantification in Single Extracellular Vesicles from Cerebrospinal Fluid and Serum of People with Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rocío Del Carmen Bravo-Miana,Jone Karmele Arizaga-Echebarria,Valeria Sabas-Ortega,Hirune Crespillo-Velasco,Alvaro Prada,Tamara Castillo-Triviño,David Otaegui

Journal of cellular and molecular medicine 26:5078-5094 PubMed36071546

2022

ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Zhang,Jiangtao Cui,Leiqun Cao,Xiaoting Tian,Yayou Miao,Yikun Wang,Shiyu Qiu,Wanxin Guo,Lifang Ma,Jinjing Xia,Xiao Zhang
View all publications
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Product promise

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