AB204131

Anti-PFKM + PFKP antibody [EPR17314]

RabMAb
Recombinant
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(21 Publications)

Rabbit Recombinant Monoclonal PFKP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.

View Alternative Names

PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C, PFKX, PFKM, ATP-PFK, PFK-M, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, Phosphohexokinase, PFK-A

9 Images

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Immunofluorescent analysis of 100% methanl-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab204131 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Immunofluorescent analysis of 100% methanl-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PFKP with ab204131 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab204131 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

PFKP was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab204131 at 1/80 dilution.

Western blot was performed from the immunoprecipitate using ab204131 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : MCF7 whole cell lysate 10ug (Input).

Lane 2 : ab204131 IP in MCF7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab204131 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

false

Supplier Data

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Observed band size: 86 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/20000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 86 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 86 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane1 : 10 seconds
Lane 2 : 1 seconds

All lanes:

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-pfkp-protein-ab132823'>ab132823</a>)

Lane 2:

DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Carrier free

Anti-PFKM + PFKP antibody [EPR17314] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17314

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKM and PFKP are key enzymes involved in glycolysis. These targets are commonly referred to as phosphofructokinase muscle (PFKM) and phosphofructokinase platelet (PFKP). PFKM has a molecular mass of approximately 85 kDa while PFKP is slightly larger at around 84 kDa. PFKM is expressed mainly in muscle tissues whereas PFKP is found in platelets and other tissues like the brain. These enzymes catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate using ATP an important regulatory step in glycolysis.

Biological function summary

PFKM and PFKP play significant roles in energy metabolism. They are part of the phosphofructokinase enzyme complex which regulates glycolytic flux. PFKM is important for muscle energy production during high-intensity exercises where anaerobic respiration predominates. PFKP on the other hand manages glycolytic rates in tissues like brain and platelets which have unique energy demands. The activity of these enzymes is controlled by various metabolites reflecting cellular energy status.

Pathways

PFKM and PFKP are central components of the glycolysis pathway a critical energy-yielding process. Glycolysis not only provides ATP but also intermediates for other pathways like the Krebs cycle. Both PFKM and PFKP interact with enzymes like aldolase and pyruvate kinase coordinating energy production. Their regulation ensures proper adaptation to varying energy requirements across different cell types and tissues.

PFKM mutations associate with glycogen storage disease type VII (Tarui's disease) where affected individuals suffer from exercise intolerance and muscle cramps. This connection highlights the importance of PFKM in muscle energy production. PFKP has been linked to cancer metabolism as it is involved in adapting glycolysis rates in tumors often called the Warburg effect. Dysregulation of these enzymes can alter metabolic processes interacting with proteins like lactate dehydrogenase contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

See full target information PFKP

Additional targets

PFKM

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 86:103812 PubMed40803248

2025

ROS-dependent localization of glycolytic enzymes to mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Pau B Esparza-Moltó,Arvind V Goswami,Süleyman Bozkurt,Christian Münch,Laura E Newman,Alexandra G Moyzis,Gladys R Rojas,Deann Guan,Jeffrey R Jones,Fred H Gage,Gerald S Shadel

Natural products and bioprospecting 15:11 PubMed39777624

2025

Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenta Tan,Shuo Fu,Yufei Wang,Bojun Hu,Guiquan Ding,Li Zhang,Wen Zhang,Guanhua Du,Junke Song

Stem cells translational medicine 14: PubMed39560969

2024

Tailoring cell therapies for diabetic metabolic phenotypes: a comparative study on the efficacy of various umbilical cord-derived cell regimens.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshu Wang,Liming Wang,Falian He,Jia Song,Jingting Qiao,Jun Qin,Li Chen,Xinguo Hou

Nature cell biology 25:1821-1832 PubMed38049604

2023

Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna M Giafaglione,Preston D Crowell,Amelie M L Delcourt,Takao Hashimoto,Sung Min Ha,Aishwarya Atmakuri,Nicholas M Nunley,Rachel M A Dang,Mao Tian,Johnny A Diaz,Elisavet Tika,Marie C Payne,Deborah L Burkhart,Dapei Li,Nora M Navone,Eva Corey,Peter S Nelson,Neil Y C Lin,Cedric Blanpain,Leigh Ellis,Paul C Boutros,Andrew S Goldstein

Nature communications 13:7578 PubMed36481721

2022

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Chunyu Yu,Xinhua Liu,Beibei Liu,Xiaodi Wu,Jiajing Wu,Dong Yan,Lulu Han,Zifan Tang,Xinyi Yuan,Jianqiu Wang,Yue Wang,Shumeng Liu,Lin Shan,Yongfeng Shang

Blood cancer discovery 4:54-77 PubMed36108149

2022

Extracellular Vesicle Secretion by Leukemia Cells In Vivo Promotes CLL Progression by Hampering Antitumor T-cell Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ernesto Gargiulo,Elodie Viry,Pablo Elías Morande,Anne Largeot,Susanne Gonder,Feng Xian,Nikolaos Ioannou,Mohaned Benzarti,Felix Bruno Kleine Borgmann,Michel Mittelbronn,Gunnar Dittmar,Petr V Nazarov,Johannes Meiser,Basile Stamatopoulos,Alan G Ramsay,Etienne Moussay,Jérôme Paggetti

Cancer communications (London, England) 42:1008-1027 PubMed35975322

2022

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Juhong Wang,Fei Shao,Yannan Yang,Wei Wang,Xueying Yang,Renda Li,Hong Cheng,Sijin Sun,Xiaoli Feng,Yibo Gao,Jie He,Zhimin Lu

Cell discovery 8:52 PubMed35641476

2022

PFKP alleviates glucose starvation-induced metabolic stress in lung cancer cells via AMPK-ACC2 dependent fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqing Chen,Li Zou,Guang Lu,Oleg Grinchuk,Lei Fang,Derrick Sek Tong Ong,Reshma Taneja,Choon-Nam Ong,Han-Ming Shen

Frontiers in pharmacology 13:863451 PubMed35450040

2022

Meldonium Ameliorates Hypoxia-Induced Lung Injury and Oxidative Stress by Regulating Platelet-Type Phosphofructokinase-Mediated Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Daohui Wang,Fengying Liu,Weijie Yang,Yangyang Sun,Xiaoning Wang,Xin Sui,Jun Yang,Qian Wang,Wenhao Song,Minmin Zhang,Zhenyu Xiao,Tian Wang,Yongan Wang,Yuan Luo

International journal of oncology 60: PubMed35029285

2022

Epigallocatechin‑3‑gallate hinders metabolic coupling to suppress colorectal cancer malignancy through targeting aerobic glycolysis in cancer‑associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhai Chen,Masaaki Nishi,Yuji Morine,Mitsuo Shimada,Takuya Tokunaga,Hideya Kashihara,Chie Takasu,Shinichiro Yamada,Yuma Wada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKM + PFKP antibody [EPR17314]

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (21)

Product promise