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Rabbit Recombinant Monoclonal PFKP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

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Images

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131), expandable thumbnail
  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131), expandable thumbnail
  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131), expandable thumbnail
  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (AB204131), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (AB204131), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/80
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Additional Targets

PFKM

Alternative names

PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C

Recommended products

Rabbit Recombinant Monoclonal PFKP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17314
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PFKM and PFKP are key enzymes involved in glycolysis. These targets are commonly referred to as phosphofructokinase muscle (PFKM) and phosphofructokinase platelet (PFKP). PFKM has a molecular mass of approximately 85 kDa while PFKP is slightly larger at around 84 kDa. PFKM is expressed mainly in muscle tissues whereas PFKP is found in platelets and other tissues like the brain. These enzymes catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate using ATP an important regulatory step in glycolysis.

Biological function summary

PFKM and PFKP play significant roles in energy metabolism. They are part of the phosphofructokinase enzyme complex which regulates glycolytic flux. PFKM is important for muscle energy production during high-intensity exercises where anaerobic respiration predominates. PFKP on the other hand manages glycolytic rates in tissues like brain and platelets which have unique energy demands. The activity of these enzymes is controlled by various metabolites reflecting cellular energy status.

Pathways

PFKM and PFKP are central components of the glycolysis pathway a critical energy-yielding process. Glycolysis not only provides ATP but also intermediates for other pathways like the Krebs cycle. Both PFKM and PFKP interact with enzymes like aldolase and pyruvate kinase coordinating energy production. Their regulation ensures proper adaptation to varying energy requirements across different cell types and tissues.

Associated diseases and disorders

PFKM mutations associate with glycogen storage disease type VII (Tarui's disease) where affected individuals suffer from exercise intolerance and muscle cramps. This connection highlights the importance of PFKM in muscle energy production. PFKP has been linked to cancer metabolism as it is involved in adapting glycolysis rates in tumors often called the Warburg effect. Dysregulation of these enzymes can alter metabolic processes interacting with proteins like lactate dehydrogenase contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/20000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 86 kDa

    Exposure time: 5s

  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Observed band size: 86 kDa

    Exposure time: 5s

  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: Rat kidney lysate at 10 µg

    Lane 4: Rat spleen lysate at 10 µg

    Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 86 kDa

    Exposure time: 5s

  • Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane1: 10 seconds
    Lane 2: 1 seconds

    All lanes: Western blot - Anti-PFKM + PFKP antibody [EPR17314] (ab204131) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human PFKP protein (Recombinant Human PFKP protein ab132823)

    Lane 2: DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    PFKP was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab204131 at 1/80 dilution.

    Western blot was performed from the immunoprecipitate using ab204131 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab204131 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab204131 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

  • Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (ab204131), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (ab204131)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

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