Rabbit Polyclonal PFKP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PFKP aa 1-50.
View Alternative Names
PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C
- IP
Supplier Data
Immunoprecipitation - Anti-PFKP antibody (AB186132)
Detection of PFKP in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186132 at 6 μg/reaction for IP. For WB detection, ab186132 was used at 1μg/ml.
Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-PFKP antibody (ab186132)
Predicted band size: 86 kDa
false
- WB
Supplier Data
Western blot - Anti-PFKP antibody (AB186132)
All lanes:
Western blot - Anti-PFKP antibody (ab186132) at 0.1 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
293T whole cell lysate at 50 µg
Lane 3:
Jurkat whole cell lysate at 50 µg
Lane 4:
TCMK-1 whole cell lysate at 50 µg
Lane 5:
NIH 3T3 whole cell lysate at 50 µg
Predicted band size: 86 kDa
true
Exposure time: 3min
Reactivity data
Have you thought about this alternative?
Anti-PFKM + PFKP antibody [EPR17314]
Why is this recommended?
We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.
Pathways
The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Nature 546:426-430 PubMed28607489
2017
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Associated Products
Alternative Product
Primary Antibodies
AB137636
Anti-PFKP antibody
primary-antibodies
pfkp-antibody-ab137636
0
(0 reviews)
Alternative Product
Primary Antibodies
AB119796
Anti-PFKP antibody [OTI1D6]
primary-antibodies
pfkp-antibody-oti1d6-ab119796
0
(0 reviews)
Alternative Product
Primary Antibodies
AB233109
Anti-PFKP antibody
primary-antibodies
pfkp-antibody-ab233109
0
(0 reviews)
Alternative Product
Primary Antibodies
AB242413
Anti-PFKM + PFKP antibody [EPR17314] - BSA and Azide free
primary-antibodies
pfkm-pfkp-antibody-epr17314-bsa-and-azide-free-ab242413
0
(0 reviews)
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com