AB233109

Anti-PFKP antibody

Name: Anti-PFKP antibody (ab233109) | Abcam
Brand: Abcam Limited
SKU: AB233109
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PFKP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFKP aa 550 to C-terminus.

View Alternative Names

PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human liver tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human brain tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PFKP antibody (AB233109)

All lanes:

Western blot - Anti-PFKP antibody (ab233109) at 5 µg/mL

All lanes:

Recombinant human PFKP protein

Predicted band size: 86 kDa

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Supplier Data

Western blot - Anti-PFKP antibody (AB233109)

All lanes:

Western blot - Anti-PFKP antibody (ab233109) at 3 µg/mL

Lane 1:

A549 (human lung carcinoma cell line) cell lysate

Lane 2:

Pig heart lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

Pig skeletal muscle lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PFKP aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKP also known as 6-phosphofructokinase platelet type functions as an important glycolytic enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate using ATP. This process shows the rate-limiting step in glycolysis. The PFKP protein weighs approximately 85 kDa. Expression occurs in various tissues with high presence in platelets and muscle tissues indicating its significant role in energy metabolism.

Biological function summary

PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.

Pathways

The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.

PFKP's alterations contribute to metabolic conditions like cancer and diabetes. Its dysregulation in cancer cells supports the Warburg effect where tumor cells prefer glycolysis. The link with metabolic proteins such as AMPK also indicates a potential role in diabetes as both disorders involve shifts in energy metabolism. Understanding PFKP's impact on these diseases helps in developing therapeutic strategies targeting metabolic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

See full target information PFKP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:361 PubMed41053001

2025

Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

KathrynAnn Odamah,Heng-Ye Man

The Journal of cell biology 221: PubMed35670764

2022

Fbxo7 promotes Cdk6 activity to inhibit PFKP and glycolysis in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Harris,Ming Yang,Christina Schmidt,Chloe Royet,Sarbjit Singh,Amarnath Natarajan,May Morris,Christian Frezza,Heike Laman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody (AB233109)

Western blot - Anti-PFKP antibody (AB233109)

Western blot - Anti-PFKP antibody (AB233109)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise