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AB224098

Anti-PFTK1 antibody

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(4 Publications)

Rabbit Polyclonal PFTK1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDK14 aa 1-200.

View Alternative Names

KIAA0834, PFTK1, CDK14, Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)

Immunohistochemical analysis of human kidney labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in tubules.

Immunocytochemistry/ Immunofluorescence - Anti-PFTK1 antibody (AB224098)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFTK1 antibody (AB224098)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PFTK1 (green) using ab224098 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)

Paraffin-embedded human cerebral cortex tissue stained for PFTK1 using ab224098 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)

Immunohistochemical analysis of human testis labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CDK14 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O94921

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dasypus novemcinctus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Titi": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFTK1 also known as PFTAIRE protein kinase 1 or CDK14 belongs to the cyclin-dependent kinase family. It has a molecular mass of approximately 36 kDa. PFTK1 is expressed in various tissues with high levels in the brain and testis. This kinase plays a role in cell cycle regulation by phosphorylating specific substrates influencing cellular processes related to proliferation and growth.
Biological function summary

PFTK1 regulates the cell cycle particularly during the G2/M transition. It does not form traditional cyclin-CDK complexes which sets it apart from other known CDKs. Instead PFTK1's activity relies on its interaction with a range of non-cyclin proteins. By aiding cell cycle progression PFTK1 impacts cellular differentiation and neuronal development which highlights its importance in neural tissues.

Pathways

PFTK1 participates in the Wnt signaling pathway and the neuronal differentiation pathway. In the Wnt pathway PFTK1 interacts with proteins that modulate beta-catenin signaling such as GSK-3β. These interactions influence transcriptional activity and cellular proliferation. The kinase's involvement in neuronal differentiation suggests its importance in neural precursor development and maturation.

PFTK1 has connections to cancer and neurodegenerative diseases. PFTK1 overexpression is often observed in cancers like gliomas suggesting its role in tumorigenesis. PFTK1 associates with Cyclin D3 in some cancers influencing tumor growth and progression. Furthermore altered PFTK1 activity impacts neuronal survival and function linking it to disorders such as Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
See full target information CDK14
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1649 PubMed39702755

2024

Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Jia,Jingjie Tian,Pingping Chen,Jing Dong,Lei Li,Danyang Chen,Jianlei Zhang,Dongjiang Liao,Zhimin He,Kai Luo

Frontiers in cell and developmental biology 9:684819 PubMed34222253

2021

Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer Regulating Tropomyosin 4.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Zhao,Mei Jiang,Yu Teng,Jie Li,Zhefeng Li,Wende Hao,Hongyu Zhao,Chenghong Yin,Wentao Yue

The journal of gene medicine 23:e3365 PubMed34031947

2021

Hsa_circRNA_102229 facilitates the progression of triple-negative breast cancer via regulating the miR-152-3p/PFTK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Du,Jianhua Zhang,Linfeng Zhang,Yingying Zhang,Yan Wang,Jingruo Li

Journal of experimental & clinical cancer research 37:219 PubMed30185212

2018

The putative tumour suppressor miR-1-3p modulates prostate cancer cell aggressiveness by repressing E2F5 and PFTK1.

Applications

Unspecified application

Species

Unspecified reactive species

Sen-Mao Li,Huan-Lei Wu,Xiao Yu,Kun Tang,Shao-Gang Wang,Zhang-Qun Ye,Jia Hu
View all publications
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