Rabbit Polyclonal PFTK1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDK14 aa 1-200.
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Publications
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- Frontiers in cell and developmental biology 9:6848192021Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer Regulating Tropomyosin 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoting Zhao,Mei Jiang,Yu Teng,Jie Li,Zhefeng Li,Wende Hao,Hongyu Zhao,Chenghong Yin,Wentao YuePubMed 34222253
- The journal of gene medicine 23:e33652021Hsa_circRNA_102229 facilitates the progression of triple-negative breast cancer via regulating the miR-152-3p/PFTK1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuang Du,Jianhua Zhang,Linfeng Zhang,Yingying Zhang,Yan Wang,Jingruo LiPubMed 34031947
- Journal of experimental & clinical cancer research 37:2192018The putative tumour suppressor miR-1-3p modulates prostate cancer cell aggressiveness by repressing E2F5 and PFTK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSen-Mao Li et. al.PubMed 30185212
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDK14 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O94921
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Common marmoset | Predicted | Predicted |
| Dasypus novemcinctus | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Titi | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Common marmoset, Xenopus tropicalis, Dasypus novemcinctus, Titi | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Common marmoset, Xenopus tropicalis, Dasypus novemcinctus, Titi | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
Alternative names
KIAA0834, PFTK1, CDK14, Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1
Recommended products
Rabbit Polyclonal PFTK1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDK14 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDK14 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O94921
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PFTK1 also known as PFTAIRE protein kinase 1 or CDK14 belongs to the cyclin-dependent kinase family. It has a molecular mass of approximately 36 kDa. PFTK1 is expressed in various tissues with high levels in the brain and testis. This kinase plays a role in cell cycle regulation by phosphorylating specific substrates influencing cellular processes related to proliferation and growth.
- Biological function summary
PFTK1 regulates the cell cycle particularly during the G2/M transition. It does not form traditional cyclin-CDK complexes which sets it apart from other known CDKs. Instead PFTK1's activity relies on its interaction with a range of non-cyclin proteins. By aiding cell cycle progression PFTK1 impacts cellular differentiation and neuronal development which highlights its importance in neural tissues.
- Pathways
PFTK1 participates in the Wnt signaling pathway and the neuronal differentiation pathway. In the Wnt pathway PFTK1 interacts with proteins that modulate beta-catenin signaling such as GSK-3β. These interactions influence transcriptional activity and cellular proliferation. The kinase's involvement in neuronal differentiation suggests its importance in neural precursor development and maturation.
- Associated diseases and disorders
PFTK1 has connections to cancer and neurodegenerative diseases. PFTK1 overexpression is often observed in cancers like gliomas suggesting its role in tumorigenesis. PFTK1 associates with Cyclin D3 in some cancers influencing tumor growth and progression. Furthermore altered PFTK1 activity impacts neuronal survival and function linking it to disorders such as Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)
Immunohistochemical analysis of human kidney labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)
Immunohistochemical analysis of human testis labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)
Paraffin-embedded human cerebral cortex tissue stained for PFTK1 using ab224098 at 1/20 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-PFTK1 antibody (ab224098)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PFTK1 (green) using ab224098 at 4 μg/ml in ICC/IF.
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