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AB239479

Anti-PGA5 antibody [C4]

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(1 Publication)

Mouse Monoclonal PEPA5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PGA5 aa 50-300.

View Alternative Names

Pepsin A-5, Pepsinogen-5, PGA5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGA5 antibody [C4] (AB239479)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGA5 antibody [C4] (AB239479)

Paraffin-embedded human stomach tissue stained for PGA5 using ab239479 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PGA5 antibody [C4] (AB239479)
  • WB

Supplier Data

Western blot - Anti-PGA5 antibody [C4] (AB239479)

All lanes:

Western blot - Anti-PGA5 antibody [C4] (ab239479) at 5 µg/mL

All lanes:

Recombinant human PGA5 protein

Predicted band size: 42 kDa

false

Western blot - Anti-PGA5 antibody [C4] (AB239479)
  • WB

Supplier Data

Western blot - Anti-PGA5 antibody [C4] (AB239479)

All lanes:

Western blot - Anti-PGA5 antibody [C4] (ab239479) at 5 µg/mL

All lanes:

Pig stomach lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C4

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PGA5 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P0DJD9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

{PGA5} also known as pepsinogen 5 or progastricsin serves as an inactive precursor that gets converted into the active enzyme pepsin A. It possesses a molecular mass of approximately 42 kDa. PGA5 is primarily expressed in the gastric mucosa particularly in the chief cells of the stomach. The enzyme exhibits an acidic pH optimum and plays a role in the initial digestion of proteins in the stomach through its proteolytic activity.
Biological function summary

The role of PGA5 centers around protein digestion within the stomach. It is part of a larger protein complex interacting with other pepsinogens and hydrolyzing proteins into peptides. This function is integral in breaking down food proteins into absorbable units assisting in nutrient uptake and the general digestive process. Its activity depends upon the acidic environment present in the stomach where it becomes activated to exert its enzymatic function.

Pathways

PGA5 holds significance in the gastric digestion pathway. It interacts with related proteins such as other pepsinogens and chymosin forming an ensemble vital for protein digestion. PGA5 also crosses paths with the aspartic protease pathway which includes related proteins like pepsin C and cathepsin E. These pathways ensure the coordinated breakdown and processing of dietary proteins reflecting PGA5's contributions to gastrointestinal physiology.

PGA5 links to gastric ulcer and peptic ulcer disorders. Changes in PGA5 expression and activity can influence the protective mucus layer contributing to ulcer development. Additionally alterations in its levels are associated with gastric carcinoma where it may influence tumor behavior. Within these contexts PGA5 interacts with proteins such as H/K-ATPase in ulcer-related pathways and matrix metalloproteinases in cancer progression highlighting its importance in both digestive and pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
See full target information PGA5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 24:83 PubMed36927357

2023

Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Rodriguez-Piñeiro,Florian Jaudas,Nikolai Klymiuk,Andrea Bähr,Gunnar C Hansson,Anna Ermund
View all publications
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Product promise

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