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Rabbit Recombinant Monoclonal PGAM1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (AB248342), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (AB248342), expandable thumbnail
  • OI-RD Scanning - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (AB248342), expandable thumbnail
  • Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (AB248342), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate (PubMed:23653202). Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate (PubMed:23653202).

Alternative names

PGAMA, CDABP0006, PGAM1, Phosphoglycerate mutase 1, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B

Recommended products

Rabbit Recombinant Monoclonal PGAM1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8042
Purification technique
Affinity purification
Dissociation constant
1.5 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248342 is the carrier-free version of Anti-PGAM1 antibody [EPR8042] ab129191.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Phosphoglycerate mutase 1 (PGAM1) also known as PGAM-M or brain PGAM is an enzyme with a molecular weight of approximately 29 kDa. PGAM1 catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate in the glycolytic pathway. This enzyme is expressed in a wide range of tissues with significant levels found in brain and muscle cells. It plays an important role in energy metabolism by facilitating the intermediate steps of glycolysis.

Biological function summary

PGAM1 acts within the glycolysis and gluconeogenesis processes interacting with other metabolic enzymes to maintain cellular energy balance. It functions as a part of the phosphoglycerate mutase enzyme complex. The activity of PGAM1 is important for optimal glycolytic flux directly linking it to energy production and regulation within the cell. By maintaining the concentrations of glycolytic intermediates PGAM1 supports various cellular processes that depend on energy generated through glycolysis.

Pathways

The activity of PGAM1 integrates glycolysis with other vital metabolic processes in the cell. One key pathway involving PGAM1 is the glycolytic pathway where it collaborates with the enzymes phosphoglycerate kinase and enolase to efficiently drive the conversion of glucose to pyruvate. Additionally PGAM1 is linked to the pentose phosphate pathway where it indirectly influences nucleotide biosynthesis and antioxidant responses by affecting the flux of metabolites into the pathway.

Associated diseases and disorders

PGAM1's involvement in energy metabolism connects it to various cancer types. Many cancer cells display elevated levels of PGAM1 which can promote enhanced aerobic glycolysis also known as the Warburg effect. This shift in metabolism is associated with increased cancer cell proliferation and survival. Furthermore PGAM1's interaction with other proteins like p53 a tumor suppressor highlights its potential role in tumorigenesis where dysregulation of these interactions can contribute to cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342), expandable thumbnail

    Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342)

    This data was developed using Anti-PGAM1 antibody [EPR8042] ab129191, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PGAM1 antibody [EPR8042] (Anti-PGAM1 antibody [EPR8042] ab129191) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: Human fetal muscle tissue lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa

  • Flow Cytometry (Intracellular) - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342)

    This data was developed using Anti-PGAM1 antibody [EPR8042] ab129191, the same antibody clone in a different buffer formulation.

    Anti-PGAM1 antibody [EPR8042] ab129191 at 1/100 dilution staining PGAM1 in permeabilized Raji cells by intracellular flow cytometry or a rabbit IgG negative control (green).

  • OI-RD Scanning - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342), expandable thumbnail

    OI-RD Scanning - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342), expandable thumbnail

    Western blot - Anti-PGAM1 antibody [EPR8042] - BSA and Azide free (ab248342)

    This data was developed using the same antibody clone in a different buffer formulation (abAB129191).

    Western blot: Anti-PGAM antibody [EPR8042] (Anti-PGAM1 antibody [EPR8042] ab129191) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PGAM1 antibody [EPR8042] ab129191 was shown to bind specifically to PGAM. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-PGAM1 antibody [EPR8042] (Anti-PGAM1 antibody [EPR8042] ab129191) at 1/1000 dilution

    Lane 1: Recombinant human PGAM2 protein (Active) Recombinant human PGAM2 protein (Active) ab219276 cell lysate at 0.1 µg

    Lane 2: Recombinant human PGAM1 protein (Active) Recombinant human PGAM1 protein (Active) ab289791 cell lysate at 0.1 µg

    Lane 3: PGAM4 Human Recombinant Protein cell lysate at 0.1 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Lane 5: A431 cell lysate at 20 µg

    Lane 6: Raji cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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