Anti-PGAM2 antibody [EPR15193(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15193(B)] (AB183027)

Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-PGAM2 antibody [EPR15193(B)] (AB183027)

Western blot : Anti-PGAM antibody [EPR15193(B)] (ab183027 ) staining at 1/10000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab183027 was shown to bind specifically to PGAM. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/10000 dilution

Lane 1:

Recombinant human PGAM2 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam2-protein-active-ab219276'>ab219276</a> cell lysate at 0.1 µg

Lane 2:

Recombinant human PGAM1 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam1-protein-active-ab289791'>ab289791</a> cell lysate at 0.1 µg

Lane 3:

PGAM4 Human Recombinant Protein cell lysate at 0.1 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

A431 cell lysate at 20 µg

Lane 6:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

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• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PGAM2 antibody [EPR15193(B)] (AB183027)

Intracellular Flow Cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution. Goatanti Rabbit IgG secondary antibody used at 1/150 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-PGAM2 antibody [EPR15193(B)] (AB183027)

Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2) Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and blocking buffer was 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Predicted band size: 29 kDa

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• WB

Supplier Data

Western blot - Anti-PGAM2 antibody [EPR15193(B)] (AB183027)

All lanes:

Western blot - Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

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